Faculty of Health Sciences, IDIBO Research Group, Rey Juan Carlos University, Madrid, Spain.
Department of Chemical and Environmental Technology, Rey Juan Carlos University, C/ Tulipán S/N Móstoles, 28933, Madrid, Spain.
J Nanobiotechnology. 2022 Dec 1;20(1):502. doi: 10.1186/s12951-022-01714-0.
Dental caries is the major biofilm-mediated oral disease in the world. The main treatment to restore caries lesions consists of the use of adhesive resin composites due to their good properties. However, the progressive degradation of the adhesive in the medium term makes possible the proliferation of cariogenic bacteria allowing secondary caries to emerge. In this study, a dental adhesive incorporating a drug delivery system based on L-arginine-containing mesoporous silica nanoparticles (MSNs) was used to release this essential amino acid as a source of basicity to neutralize the harmful acidic conditions that mediate the development of dental secondary caries. The in vitro and bacterial culture experiments proved that L-arginine was released in a sustained way from MSNs and diffused out from the dental adhesive, effectively contributing to the reduction of the bacterial strains Streptococcus mutans and Lactobacillus casei. Furthermore, the mechanical and bonding properties of the dental adhesive did not change significantly after the incorporation of L-arginine-containing MSNs. These results are yielding glimmers of promise for the cost-effective prevention of secondary caries.
龋齿是世界上主要的生物膜介导的口腔疾病。修复龋齿病变的主要治疗方法是使用具有良好性能的黏附性树脂复合材料。然而，黏合剂在中期的逐渐降解使得致龋细菌得以增殖，从而导致继发性龋齿的出现。在这项研究中，使用了一种含有基于含 L-精氨酸的介孔硅纳米粒子（MSNs）的药物输送系统的牙科黏合剂，将这种必需氨基酸释放出来作为碱性物质的来源，以中和介导牙继发龋发展的有害酸性条件。体外和细菌培养实验证明，L-精氨酸从 MSNs 中持续释放并从牙科黏合剂中扩散出来，有效地有助于减少变形链球菌和干酪乳杆菌的菌株数量。此外，在掺入含 L-精氨酸的 MSNs 后，牙科黏合剂的机械和黏合性能没有发生显著变化。这些结果为经济有效地预防继发性龋齿带来了一线希望。
