Department of Orthodontics, Dental Medical Center, China-Japan Friendship Hospital, Beijing 100029, China.
Department of Orthodontics, Dental Medical Center, China-Japan Friendship Hospital, Beijing 100029, China; Peking Union Medical College Hospital, Peking Union Medical College, Beijing 100193, China.
J Dent. 2024 Jun;145:104992. doi: 10.1016/j.jdent.2024.104992. Epub 2024 Apr 9.
The objective of this study was to synthesize arginine loaded mesoporous silica nanoparticles (Arg@MSNs), develop a novel orthodontic adhesive using Arg@MSNs as modifiers, and investigate the adhesive performance, antibacterial activity, and biocompatibility.
Arg@MSNs were synthesized by immobilizing arginine into MSNs and characterized using transmission electron microscope (TEM), dynamic light scattering (DLS), and Fourier Transform Infrared Spectrometer (FT-IR). Arg@MSNs were incorporated into Transbond XT adhesive with different mass fraction to form functional adhesives. The degree of conversion (DC), arginine release behavior, adhesive performance, antibacterial activity against Streptococcus mutans biofilm, and cytotoxicity were comprehensively evaluated.
TEM, DLS, and FT-IR characterizations confirmed the successful preparation of Arg@MSNs. The incorporation of Arg@MSNs did not significantly affect DC and exhibited clinically acceptable bonding strength. Compared to the commercial control, the Arg@MSNs modified adhesives greatly suppressed the metabolic activity and polysaccharide production while increased the biofilm pH values. The cell counting kit (CCK)-8 test indicated no cytotoxicity.
The novel orthodontic adhesive containing Arg@MSNs exhibited significantly enhanced antibacterial activities and inhibitory effects on acid production compared to the commercial adhesive without compromising their bonding strength or biocompatibility.
The novel orthodontic adhesive containing Arg@MSNs exhibits potential clinical benefits in preventing demineralization of enamel surfaces around or beneath orthodontic brackets due to its enhanced antibacterial activities and acid-producing inhibitory effects.
本研究旨在合成精氨酸负载介孔硅纳米粒子（Arg@MSNs），开发一种新型的正畸用胶粘剂，以 Arg@MSNs 作为改性剂，研究其粘结性能、抗菌活性和生物相容性。
通过将精氨酸固定在 MSNs 上合成 Arg@MSNs，并用透射电子显微镜（TEM）、动态光散射（DLS）和傅里叶变换红外光谱仪（FT-IR）进行表征。将 Arg@MSNs 以不同的质量分数掺入 Transbond XT 胶粘剂中，形成功能性胶粘剂。综合评估转化率（DC）、精氨酸释放行为、粘结性能、抗变形链球菌生物膜的抗菌活性和细胞毒性。
TEM、DLS 和 FT-IR 特征分析证实了 Arg@MSNs 的成功制备。Arg@MSNs 的掺入对 DC 没有显著影响，且具有临床可接受的粘结强度。与商业对照相比，Arg@MSNs 改性的胶粘剂显著抑制了代谢活性和多糖的产生，同时增加了生物膜的 pH 值。细胞计数试剂盒（CCK-8）试验表明无细胞毒性。
与商业胶粘剂相比，新型含 Arg@MSNs 的正畸用胶粘剂具有显著增强的抗菌活性和抑制产酸作用，同时不影响其粘结强度和生物相容性。
新型含 Arg@MSNs 的正畸用胶粘剂由于其增强的抗菌活性和抑制产酸作用，在预防正畸托槽周围或下方牙釉质表面脱矿方面具有潜在的临床益处。
