Ring J, Duswald K H, Bachmann T, von Scheel J, Stephan W, Brendel W
Res Exp Med (Berl). 1978 Sep 25;173(3):209-18. doi: 10.1007/BF01851492.
Dog IgG was produced by fractionation procedures used for the production of clinically used i.v. gammaglobulins. Chemical modification of dog IgG was done by pepsin or beta-propiolactone treatment. The intravascular half-life of beta-propiolactone IgG was 8.5 +/- 2.1 days compared to 4.5 +/- 1.6 days of pepsin treated IgG. Tissue concentrations of radioactive labelled beta-propiolactone IgG were generally higher than of pepsin digested IgG. Pepsin treated Igg was degraded to a significantly higher extent (26% of the administered radioactivity was bound to fragments smaller than 6000 MW after three days) than beta-propiolactone IgG (9% fragments after the same interval, P less than 0.001). It is concluded that the short intravascular half-life of pepsin IgG cannot be explained by increased extravascular filling, but is due to rapid degradation and excretion via the kidneys. There was no obvious difference in elimination and organ distribution between standard and beta-propiolactone IgG.
犬IgG是通过用于生产临床使用的静脉注射免疫球蛋白的分级分离程序制备的。犬IgG的化学修饰通过胃蛋白酶或β-丙内酯处理完成。与胃蛋白酶处理的IgG的4.5±1.6天相比，β-丙内酯IgG的血管内半衰期为8.5±2.1天。放射性标记的β-丙内酯IgG的组织浓度通常高于胃蛋白酶消化的IgG。胃蛋白酶处理的IgG降解程度明显更高（三天后，26%的给药放射性与分子量小于6000MW的片段结合），高于β-丙内酯IgG（相同间隔后为9%的片段，P<0.001）。得出的结论是，胃蛋白酶IgG血管内半衰期短不能用血管外充盈增加来解释，而是由于通过肾脏快速降解和排泄。标准IgG和β-丙内酯IgG在消除和器官分布方面没有明显差异。
