Victor Babes University of Medicine and Pharmacy Timisoara, Biochemistry and Pharmacology Department, Biochemistry Discipline, Eftimie Murgu Sq. no. 2, 300041, Timisoara, Romania.
Victor Babes University of Medicine and Pharmacy Timisoara, Faculty of Medicine, Eftimie Murgu Sq. no. 2, 300041, Timisoara, Romania.
Biomed Res Int. 2022 Nov 22;2022:4293172. doi: 10.1155/2022/4293172. eCollection 2022.
Glycosphingolipids (GSLs) play numerous roles in cellular processes, including cell proliferation, apoptosis, inflammation, and cell signaling. Alteration of the GSLs metabolism leads to the accumulation of particular species of GSLs, which can lead to various pathologies, including carcinogenesis and metastasis; in essence, all neoplasms are characterized by the synthesis and aberrant organization of GSLs expressed on the cell surface. Secondary brain tumors make up the majority of intracranial cancers and generally present an unfavorable prognosis. In the present work, a native GSL mixture extracted and purified from a secondary brain tumor with primary pulmonary origin was obtained through extraction and purification and analyzed by MALDI TOF mass spectrometry. Research in the field of lipidomics could offer new data for the understanding of brain tropism and metastatic pathways, by studying the glycolipid molecules involved in the process of metastasis in general and in the production of brain metastases in particular. This could shed new light on the pattern of lipid glycosylation in secondary brain tumors, with a great impact on the effectiveness of cancer therapies, which could be adapted to the specific molecular pattern of the tumor.
糖脂（GSLs）在细胞过程中发挥着多种作用，包括细胞增殖、凋亡、炎症和细胞信号转导。GSL 代谢的改变导致特定种类的 GSL 积累，这可能导致各种病理学，包括癌发生和转移；从本质上讲，所有的肿瘤都以细胞表面表达的 GSL 的合成和异常组织为特征。继发性脑肿瘤构成了颅内癌症的大部分，通常预后不良。在本工作中，通过提取和纯化，从原发性肺起源的继发性脑肿瘤中提取和纯化了一种天然 GSL 混合物，并通过 MALDI-TOF 质谱进行了分析。脂质组学领域的研究通过研究参与转移过程的糖脂分子，特别是参与脑转移形成的糖脂分子，可以为了解脑趋向性和转移途径提供新的数据。这可能会揭示继发性脑肿瘤中糖基化脂质的模式，对癌症治疗的效果有很大的影响，这些治疗可以适应肿瘤的特定分子模式。
