Masruri Batol, Alimohammadi Iraj, Abyaz Mohammad Reza, Moghadasi Nargess, Yekzamani Parvaneh, Mohammadzadeh Asl Baharak, Asadmasjedi Nooshin, Ashtarinezhad Azadeh
Student Research Committee, School of Health, Iran University of Medical Sciences, Tehran, Iran.
Department of Occupational Health Engineering, School of Health, Iran University of Medical Sciences, Tehran, Iran.
J Family Reprod Health. 2022 Jun;16(2):132-138. doi: 10.18502/jfrh.v16i2.9483.
Environmental stressors such as physical and chemical factors can have a destructive testicular function. The present study aimed to determine the effect of concomitant exposure to lead and noise on testicular tissue in male rats. Twenty male Wistar rats (250-300 g, 12-13wk) were divided into four groups (n=5/each): 1) Control group, 2) Lead group treated with 4 mg/kg lead acetate by gavage for 30 days, 3) Noise group exposed to 4 kHz octave band at 105 dBA for eight hrs./ day for 30 days, and 4) the exposed group to lead plus noise concurrently. The testes' weight was measured, and testes abnormalities were assessed after staining with Hematoxylin and Eosin. The results showed that the weight of testes in experimental groups was significantly decreased compared with the control group (p<0.0001). Also indicated edema, degeneration and necrotic cell debris in the lumen, congestion and atrophy of seminiferous tubule in rat testes tissue due to sub-acute exposure to lead and noise. Exposure to 105 dB and lead can cause damage to the seminiferous tubules, intubation edema, and testicular weight loss compared to control. We also found that simultaneous exposure to noise and lead could have more detrimental effects on testicular histology and weight than others.
物理和化学因素等环境应激源会对睾丸功能产生破坏作用。本研究旨在确定同时暴露于铅和噪声对雄性大鼠睾丸组织的影响。将20只雄性Wistar大鼠（体重250 - 300克，12 - 13周龄）分为四组（每组n = 5）：1）对照组；2）铅组，通过灌胃给予4毫克/千克醋酸铅，持续30天；3）噪声组，每天暴露于105分贝A声级的4千赫倍频程带噪声8小时，持续30天；4）铅加噪声同时暴露组。测量睾丸重量，并用苏木精和伊红染色后评估睾丸异常情况。结果显示，与对照组相比，实验组睾丸重量显著降低（p < 0.0001）。亚急性暴露于铅和噪声还导致大鼠睾丸组织出现管腔水肿、变性和坏死细胞碎片、生精小管充血和萎缩。与对照组相比，暴露于105分贝的噪声和铅会导致生精小管损伤、管腔水肿和睾丸重量减轻。我们还发现，同时暴露于噪声和铅对睾丸组织学和重量的有害影响比其他情况更大。
