Shabani Manoochehr, Solhjoo Kavous, Taghipour Ali, Davami Mohammad Hasan, Rahmanian Ehsan, Karami Saina, Nikkhoo Amir Mohammad, Hooshmand Farhang
Zoonoses Research Center, Jahrom University of Medical Sciences, Jahrom, Iran.
Department of Medical Parasitology and Mycology, School of Medicine, Jahrom University of Medical Sciences, Jahrom, Iran.
J Parasit Dis. 2022 Dec;46(4):1127-1132. doi: 10.1007/s12639-022-01535-w. Epub 2022 Oct 3.
Microsporidia spp. are recognized emerging zoonotic microorganisms in immunocompromised and immunocompetent populations. Therefore, we conducted a study to investigate the occurrence, sociodemographic and risk factors of microsporidia spp. infection using microscopic and molecular techniques in children with diarrhea in Jahrom city (Fars province, southern Iran). Stool samples were gathered from 395 children aged ≤ 14 years between January 2017 and January 2018. Next, a questionnaire includes the age, sex, living area, immune system status, breast milk consumption, contact with animals, and type of water source was completed for all children. For microscopic assessment, modified acid-fast trichrome staining was applied for detection of microsporidia spores. Furthermore, DNA extraction and PCR were accomplished for all samples. A total of 15 (3.79%; 95% confidence interval [CI] 2.14-6.19) and 8 (2.02%; 95% CI 0.88-3.95) samples were positive for microsporidia infection by microscopic and molecular techniques, respectively. Although none of the sociodemographic and risk factors were significant ( value > 0.05), the prevalence of this infection was higher in some variables (e.g., rurality, contact with animals and well water consumption). Three random samples were sequenced that all isolates belonged to . Cognizance of microsporidia occurrence, risk factors, and disease disorders for the health specialists, physicians, children's families are important for appropriately control the infection.
微孢子虫属是免疫功能低下和免疫功能正常人群中公认的新兴人畜共患微生物。因此，我们开展了一项研究，采用显微镜和分子技术调查伊朗南部法尔斯省贾赫罗姆市腹泻儿童中微孢子虫属感染的发生率、社会人口统计学特征和危险因素。在2017年1月至2018年1月期间，从395名年龄≤14岁的儿童中采集粪便样本。接下来，为所有儿童填写了一份问卷，内容包括年龄、性别、居住地区、免疫系统状况、母乳喂养情况、与动物接触情况以及水源类型。对于显微镜评估，采用改良抗酸三色染色法检测微孢子虫孢子。此外，对所有样本进行了DNA提取和聚合酶链反应（PCR）。通过显微镜和分子技术检测，分别有15份（3.79%；95%置信区间[CI]2.14 - 6.19）和8份（2.02%；95%CI 0.88 - 3.95）样本微孢子虫感染呈阳性。尽管社会人口统计学特征和危险因素均无显著差异（P值>0.05），但在某些变量（如农村地区、与动物接触以及饮用井水）中，这种感染的患病率较高。对三个随机样本进行了测序，所有分离株均属于。卫生专家、医生和儿童家庭了解微孢子虫的发生情况、危险因素和疾病紊乱情况对于适当控制感染很重要。
