高压氧治疗诱导脊髓损伤大鼠模型中的分子和通路变化：蛋白质组学分析

Li Zhuo, Hou Xiaomin, Liu Xuehua, Ma Linlin, Tan Jiewen

Department of Rehabilitation Medicine, Guangzhou Xinhua University, Guangzhou, China.

Hyperbaric Oxygen Department, Shenzhen People's Hospital, Shenzhen, China.

Dose Response. 2022 Nov 24;20(4):15593258221141579. doi: 10.1177/15593258221141579. eCollection 2022 Oct-Dec.

Hyperbaric Oxygen Therapy (HBOT) has definitive therapeutic effects on spinal cord injury (SCI), but its mechanism of action is still unclear. Here, we've conducted a systemic proteomic analysis to identify differentially expressed proteins (DEPs) between SCI rats and HBOT + SCI rats. The function clustering analysis showed that the top enriched pathways of DEPs include oxygen transport activity, oxygen binding, and regulation of T cell proliferation. The results of functional and signal pathway analyses indicated that metabolic pathways, thermogenesis, LXR/RXR activation, acute phase response signaling, and the intrinsic prothrombin pathway in the SCI + HBOT group was higher than SCI group.

高压氧疗法（HBOT）对脊髓损伤（SCI）具有确切的治疗效果，但其作用机制仍不清楚。在此，我们进行了一项系统性蛋白质组学分析，以鉴定SCI大鼠和HBOT + SCI大鼠之间的差异表达蛋白（DEP）。功能聚类分析表明，DEP的顶级富集途径包括氧转运活性、氧结合和T细胞增殖调节。功能和信号通路分析结果表明，SCI + HBOT组的代谢途径、产热、LXR/RXR激活、急性期反应信号和内源性凝血酶原途径均高于SCI组。