导向生物测定法从苹果属植物（Malus prunifolia (Willd.) Borkh.）中制备抗氧化、抗炎活性部位。

Bioassay-guided preparation of antioxidant, anti-inflammatory active fraction from crabapples (Malus prunifolia (Willd.) Borkh.).

机构信息

State Key Laboratory Basis of Xinjiang Indigenous Medicinal Plants Resource Utilization and Key Laboratory of Plants Resources and Chemistry of Arid Zone, Xinjiang Technical Institute of Physics and Chemistry, Chinese Academy of Sciences, South Beijing Road 40-1, Urumqi 830011, Xinjiang, PR China; University of Chinese Academy of Sciences, Beijing 100039, PR China.

出版信息

Food Chem. 2023 Apr 16;406:135091. doi: 10.1016/j.foodchem.2022.135091. Epub 2022 Nov 26.

DOI:10.1016/j.foodchem.2022.135091

PMID:36459796

Abstract

The aim of current study was to optimize the extraction process and purification of main components (MC) to obtain high antioxidant, anti-inflammatory effective fractions from crabapple (Malus prunifolia (Willd.) Borkh.). The effects of three variables including ethanol concentration A, solid-liquid ratio A, extraction temperature A were investigated and optimized by response surface methodology (RSM) coupled with Box-Behnken design (BBD). The adsorption/desorption characteristics of MC on the five types of macroporous resins were investigated. According to batch adsorption test, HPD-300 resins were selected for kinetics. The adsorption mechanism showed that the process was appropriate by pseudo-second-order kinetics model, and purification parameters of MC were optimized through adsorption/desorption experiments with the column packed by HPD-300 resin. The effective fractions were obviously superior to other fractions according to DPPH, ABTS, COX-2 and 15-LOX radical scavenging. This work implies that the purified active fraction with high contents of antioxidants and anti-inflammatory compounds from crabapple might be potential source for natural products and food industries.

摘要

本研究旨在优化提取工艺和主要成分（MC）的纯化，从海棠果（Malus prunifolia（Willd.）Borkh.）中获得高抗氧化、抗炎有效部位。采用响应面法（RSM）结合 Box-Behnken 设计（BBD）考察了乙醇浓度 A、固液比 A、提取温度 A 三个变量的影响，并进行了优化。考察了 MC 在五种大孔树脂上的吸附/解吸特性。根据间歇吸附试验，选择 HPD-300 树脂进行动力学研究。吸附动力学模型符合准二级动力学模型，且通过 HPD-300 树脂填充柱的吸附/解吸实验对 MC 的纯化参数进行了优化。根据 DPPH、ABTS、COX-2 和 15-LOX 自由基清除实验，有效部位明显优于其他部位。该研究表明，从海棠果中提取的富含抗氧化剂和抗炎化合物的纯化活性部位可能是天然产物和食品工业的潜在来源。