Supawat Benjamaporn, Vorasiripreecha Watcharit, Wattanapongpitak Sakornniya, Kothan Suchart, Tungjai Montree
Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand; Radiation Research and Medical Imaging Unit, Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand.
Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai, 50200, Thailand.
Appl Radiat Isot. 2023 Feb;192:110577. doi: 10.1016/j.apradiso.2022.110577. Epub 2022 Nov 25.
This current study was designed to determine the effects of in vitro exposure to radioactive cesium-137 on human blood components. Whole blood samples were given a radiation dose of 0.02, 0.05, 0.1, 0.2, and 0.3 mGy of gamma radiation using a Cs radioactive standard source. The whole blood samples that were exposed to 0 mGy served as sham-controls. The spectrofluoroscopic technique was used to determine the autofluorescence spectrum of protein in plasma or red blood cells by using excitation wavelength and range of emission wavelengths at 280 nm and 300-550 nm, respectively. The hemolysis of red blood cells was evaluated by determination of the release of hemoglobin from the red blood cells to the supernatant. Complete blood counts were also determined in whole blood. The results showed that there was no change in the ratio of fluorescence emission intensity at 340 nm of wavelength of protein extract from irradiated whole blood or red blood cells compared to the corresponding non-irradiated control. The hemolysis value did not change in irradiated whole blood when compared to the corresponding non-irradiated group. In addition, complete blood count values in irradiated groups did not differ from non-irradiated group. These current results suggested that there were no harmful effects of the low-dose gamma radiation from radioactive Cs on blood components when human whole blood was exposed to gamma radiation in an in vitro condition.
本项研究旨在确定体外暴露于放射性铯 - 137对人体血液成分的影响。使用铯放射性标准源，对全血样本给予0.02、0.05、0.1、0.2和0.3毫戈瑞的γ辐射剂量。暴露于0毫戈瑞的全血样本作为假对照。采用荧光光谱技术，分别使用280纳米的激发波长和300 - 550纳米的发射波长范围，测定血浆或红细胞中蛋白质的自发荧光光谱。通过测定血红蛋白从红细胞释放到上清液中的量来评估红细胞的溶血情况。还对全血进行了全血细胞计数。结果表明，与相应的未辐照对照相比，辐照全血或红细胞的蛋白质提取物在波长340纳米处的荧光发射强度比值没有变化。与相应的未辐照组相比，辐照全血的溶血值没有变化。此外，辐照组的全血细胞计数值与未辐照组没有差异。目前的这些结果表明，当人体全血在体外条件下暴露于γ辐射时，放射性铯产生的低剂量γ辐射对血液成分没有有害影响。
