Supawat Benjamaporn, Homnuan Panumas, Kanthawong Natthawan, Semrasa Niyada, Tima Singkome, Kothan Suchart, Udomtanakunchai Chatchanok, Tungjai Montree
Department of Radiologic Technology, Faculty of Associated Medical Sciences, Chiang Mai University, Chiang Mai 50200, Thailand.
Center of Radiation Research and Medical Imaging, Department of Radiologic Technology, Chiang Mai University, Chiang Mai 50200, Thailand.
Mol Clin Oncol. 2021 Apr;14(4):74. doi: 10.3892/mco.2021.2236. Epub 2021 Feb 23.
High-dose radiation is deleterious to cells or tissues. However, the health risks of exposure to low-dose radiation remain unclear. The present study aimed to investigate the biological responses of low-dose gamma-ray exposure to normal red blood cells (RBCs) and erythroleukemia (K562 and K562/Dox) cancer cells. Cells were given a low dose of 0.03, 0.05 and 0.1 mGy of Cs gamma-rays (at a dose rate of 0.001 Gy/min) under conditions. Cells exposed to 0 Gy served as controls. Hemolysis and reactive oxygen species (ROS) were measured in exposed RBCs following exposure to low-dose gamma-rays. In addition, complete blood count (CBC) parameters were determined in irradiated whole blood. For irradiated K562 and K562/Dox cancer cells, ROS and mitochondrial activity were measured at 0, 30, 60 and 120 post-irradiation times. The results showed no change in the percentage of ROS and hemolysis in irradiated RBCs. The data indicated no perturbation in the CBC parameters in irradiated whole blood. By contrast, statistically significant dose-dependent increases in the percentage of ROS and decreases in the mitochondrial activity in the K562 and K562/Dox cancer cells were observed from 0 min up to 120 min post-irradiation. These findings concluded that there were differences in biological responses in normal cells (RBCs) and cancer cells (K562 and K562/Dox) to low-dose gamma-rays when cells were irradiated under conditions.
高剂量辐射对细胞或组织有害。然而，低剂量辐射暴露的健康风险仍不明确。本研究旨在调查低剂量γ射线暴露对正常红细胞（RBCs）和红白血病（K562和K562/Dox）癌细胞的生物学反应。在特定条件下，细胞接受0.03、0.05和0.1 mGy的低剂量铯γ射线（剂量率为0.001 Gy/min）。暴露于0 Gy的细胞作为对照。在低剂量γ射线照射后，测量暴露的红细胞中的溶血和活性氧（ROS）。此外，测定辐照全血中的全血细胞计数（CBC）参数。对于辐照后的K562和K562/Dox癌细胞，在辐照后0、30、60和120分钟测量ROS和线粒体活性。结果显示，辐照红细胞中ROS百分比和溶血率无变化。数据表明辐照全血中的CBC参数无扰动。相比之下，在辐照后0分钟至120分钟期间，观察到K562和K562/Dox癌细胞中ROS百分比有统计学意义的剂量依赖性增加，线粒体活性降低。这些发现得出结论，当在特定条件下照射细胞时，正常细胞（RBCs）和癌细胞（K562和K562/Dox）对低剂量γ射线的生物学反应存在差异。
