Kim HanSol, Jang Eun Jo, Sankpal Narendra V, Patel Madhumita, Patel Rajkumar
Bio-Convergence (BC), Integrated Science and Engineering Division (ISED), Underwood International College, Yonsei University, 85 Songdogwahak-ro, Yeonsu-gu, Incheon, 21983, South Korea.
Nano Science and Engineering, Integrated Science and Engineering Division (ISED), Underwood International College, Yonsei University, Songdogwahak-ro, Yeonsu-gu, Incheon, 21983, South Korea.
Macromol Biosci. 2023 Mar;23(3):e2200346. doi: 10.1002/mabi.202200346. Epub 2022 Dec 18.
Over the years, scientists have studied the behavior and anatomy of many animals to understand the own species. However, despite the continuous efforts, it is often difficult to know for certain how the brain works due to the differences between the brains of animals and the human brain. While the use of animal models for research continues, the origin of human cognition and neurological disorders needs further elucidation. To that end, in vitro organoids that exhibit in vivo characteristics of the human brain have been recently developed. These brain-like organoids enable researchers to dive deeper into understanding the human brain, its neurological structures, and the causes of neurological pathologies. This paper reviews the recent developments in the regeneration of brain-like organoids using Matrigel and other alternatives. Further, gel-free methods that may enhance the regeneration process of organoids are discussed. Finally, the vascularized brain organoid growth and development in both in vitro and in vivo conditions are detailed.
多年来，科学家们研究了许多动物的行为和解剖结构，以了解自身物种。然而，尽管不断努力，但由于动物大脑与人类大脑存在差异，往往很难确切知道大脑是如何工作的。虽然动物模型在研究中仍在继续使用，但人类认知和神经疾病的起源仍需进一步阐明。为此，最近已开发出具有人类大脑体内特征的体外类器官。这些类脑器官使研究人员能够更深入地了解人类大脑、其神经结构以及神经病理学的病因。本文综述了使用基质胶和其他替代物在类脑器官再生方面的最新进展。此外，还讨论了可能增强类器官再生过程的无凝胶方法。最后，详细介绍了体外和体内条件下血管化脑类器官的生长和发育情况。
