PLGA 纳米粒与多糖 1 联合提高传染性法氏囊病病毒 VP2 蛋白的免疫原性。

PLGA nanoparticle with polysaccharide 1 increased the immunogenicity of infectious bursal disease virus VP2 protein.

Institute of Traditional South Chinese Veterinary Pharmacology, College of Animal Science and Technology, Hainan University, Haikou, P R China.

Institute of Traditional Chinese Veterinary Medicine, College of Veterinary Medicine, Nanjing Agricultural University, Nanjing, P R China.

Br Poult Sci. 2023 Apr;64(2):176-184. doi: 10.1080/00071668.2022.2154639. Epub 2023 Feb 8.

Abstract

The purpose of this study was to create ALP1-VP2-PLGA nanoparticle (AVPN) and to study the immunogenicity of AVPN. AVPN was prepared and observed by scanning and transmission electron microscopies.2. Chickens were divided into five groups and vaccinated with normal saline, VP2 protein, ALP1 and VP2 protein, AVPN or PLGA, respectively. After 28 days, the immune organ indexes were calculated; specific antibody levels in blood were detected by enzyme-linked immunosorbent assay (ELISA). Additionally, the spleen and bursa of Fabricius were determined by HE staining, immunological cytokine mRNA levels in bursa of Fabricius were detected by qPCR andchicken body weight was determined.3. The results indicated that AVPN was a spherical nanoparticle with a diameter of about 85 nm. It increased bursal indexes and IBDV-specific antibody levels and promoted the expression of IL-2 mRNA in blood and TNF-α and IgG mRNA in bursa of Fabricius. This promoted growth.4. This study suggested that AVPN can increase immunogenicity of VP2 protein, and it could possibly be used as an IBDV subunit vaccine.

摘要

本研究旨在制备 ALP1-VP2-PLGA 纳米粒（AVPN）并研究其免疫原性。通过扫描和透射电子显微镜观察 AVPN 的制备和形态。
将鸡分为五组，分别用生理盐水、VP2 蛋白、ALP1 和 VP2 蛋白、AVPN 或 PLGA 免疫。28 天后，计算免疫器官指数；通过酶联免疫吸附试验（ELISA）检测血清中特异性抗体水平。此外，通过 HE 染色检测法氏囊的组织学变化，通过 qPCR 检测法氏囊中免疫细胞因子 mRNA 水平，并测定鸡体重。
结果表明，AVPN 是一种约 85nm 的球形纳米颗粒。它增加了法氏囊指数和 IBDV 特异性抗体水平，并促进了血液中 IL-2 mRNA 和法氏囊中 TNF-α和 IgG mRNA 的表达，从而促进了生长。
本研究表明，AVPN 可以提高 VP2 蛋白的免疫原性，有可能作为 IBDV 亚单位疫苗使用。