Department of Periodontics, School of Dentistry, Dental Implants Research Center, Hamadan University of Medical Sciences, Hamadan, Iran.
Department of Photodynamic, Medical Laser Research Center, Yara Institute, ACECR, Tehran, Iran.
J Biomol Struct Dyn. 2023 Nov;41(19):10051-10058. doi: 10.1080/07391102.2022.2152869. Epub 2022 Dec 5.
Reconstruction of lost tooth structures and the periodontium with the help of tissue engineering has found a special place in dentistry in recent years with reports of great therapeutic success. Stem cells from the periodontal ligament have the potential for high differentiation into the bone and periodontal ligament cells and are therefore a suit candidate for regenerative therapies of the periodontium and other tissues. In this regard, the use of photobiomodulation on these cells by light irradiation can be effective in increasing the efficiency of these regenerative methods. The effect of red and near-infrared lasers was investigated in pulsed and continuous modes on the cell viability, ROS production and the cell cycle of Periodontal Ligament Stem cells (PDLSCs) using MTT assay and flowcytometry techniques. The result shows that both red and near-infra-red (NIR) irradiations at 3 J/cm maintain cell viability. ROS generation assay indicated that in PDL stem cells irradiated with NIR laser (940 nm), ROS production was greater than in the red (660 nm) irradiated groups. Cell cycle analysis revealed that NIR irradiation can enhance the proportion of S-phase cells and declinedecline the proportion of G1-phase cells compared to the red laser irradiation groups. Moreover, this enhancement was greater in the pulsed group compared to the continuous mode group. Overall, the current study results showed that photobiomodulation can support the cell viability of PDLSCs and could affect the ROS production and cell cycle. This effect was more with 940 nm (NIR) irradiation pulsed mode compared to 660 nm (red).Communicated by Ramaswamy H. Sarma.
近年来，组织工程技术在帮助牙科领域重建缺失的牙齿结构和牙周组织方面取得了巨大的成功，得到了广泛的关注。牙周膜干细胞具有向骨和牙周膜细胞高度分化的潜力，因此是牙周组织和其他组织再生治疗的合适候选细胞。在这方面，通过光照射对这些细胞进行光生物调节可以有效地提高这些再生方法的效率。本研究采用 MTT 检测法和流式细胞术技术，研究了脉冲和连续模式的红色和近红外（NIR）激光对牙周膜干细胞（PDLSCs）细胞活力、ROS 产生和细胞周期的影响。结果表明，3J/cm 的红光和近红外光（NIR）均能维持细胞活力。ROS 产生实验表明，与红光（660nm）照射组相比，NIR 激光（940nm）照射的 PDL 干细胞产生的 ROS 更多。细胞周期分析表明，与红光照射组相比，NIR 照射可以增加 S 期细胞的比例，降低 G1 期细胞的比例。此外，与连续模式组相比，脉冲模式组的增强效果更大。总的来说，本研究结果表明，光生物调节可以支持 PDLSCs 的细胞活力，并可能影响 ROS 产生和细胞周期。与 660nm（红光）相比，940nm（NIR）照射的脉冲模式效果更好。
