Department of Oncology, Xiangya Hospital, Central South University, Changsha, 410011, Hunan Province, China.
Department of Radiology, The Second Xiangya Hospital, Central South University, Changsha, 410011, Hunan Province, China.
Sci Rep. 2022 Dec 5;12(1):21014. doi: 10.1038/s41598-022-25318-8.
Partial bile duct ligation (pBDL) is considered a well-tolerated cholestatic model. Magnetic resonance imaging (MRI) is one of the most widely used tools in noninvasive imaging. However, no systematic studies have reported the possible effects of repeated MRI assessments in the pBDL model. Sixty BALB/C mice were investigated. MRI images of each mouse were recorded once every 2 weeks for 6 weeks after pBDL or sham surgery. The reproducibility of the pBDL model and the reliability of MRI were examined by behavioral, physiological, biochemical, and pathological parameters. The mice showed no alterations on behavioral and physiological tests (P > 0.05) at 2, 4, and 6 weeks after pBDL. Repeated general anesthesia did not result in any impairment after pBDL (P > 0.05). The behavioral and biochemical parameters were not affected by repeated MRIs or repeated contrast-enhanced MRIs (P > 0.05). Pathological staining showed the homogeneous formation of collagenous fiber in the pBDL mice and did not indicate any influence of repeated contrast-enhanced MRI on the number of inflammatory cells or fibrotic formation (P > 0.05). Thus, pBDL is a reproducible model with many advantages for animal welfare and scientific research. Additionally, MRI, as a safe tool for longitudinal evaluation and is well tolerated in mice with cholestasis.
部分胆管结扎术（pBDL）被认为是一种耐受性良好的胆汁淤积模型。磁共振成像（MRI）是无创成像中最广泛使用的工具之一。然而，没有系统的研究报道过在 pBDL 模型中重复 MRI 评估的可能影响。本研究共纳入 60 只 BALB/C 小鼠。在 pBDL 或假手术后的 6 周内，每 2 周对每只小鼠进行一次 MRI 图像记录。通过行为、生理、生化和病理参数来检查 pBDL 模型的可重复性和 MRI 的可靠性。pBDL 后 2、4 和 6 周，小鼠在行为和生理测试中没有任何改变（P>0.05）。反复全身麻醉后，pBDL 也不会造成任何损伤（P>0.05）。行为和生化参数不受重复 MRI 或重复对比增强 MRI 的影响（P>0.05）。病理染色显示 pBDL 小鼠胶原纤维均匀形成，且重复对比增强 MRI 对炎症细胞数量或纤维化形成没有任何影响（P>0.05）。因此，pBDL 是一种具有许多动物福利和科学研究优势的可重复模型。此外，MRI 作为一种安全的纵向评估工具，在胆汁淤积的小鼠中具有良好的耐受性。
