Yokota Shinichiro, Ono Yoshihiro, Nakao Toshimasa, Zhang Peng, Michalopoulos George K, Khan Zahida
Department of Surgery, Jichi Medical University; Department of Surgery, University of Pittsburgh School of Medicine.
Department of Surgery, University of Pittsburgh School of Medicine.
J Vis Exp. 2018 Mar 28(133):56930. doi: 10.3791/56930.
In rodents, complete bile duct ligation (cBDL) of the common bile duct is an established surgical technique for studying obstructive cholestasis and bile duct proliferation. However, long-term experiments can lead to increased morbidity and mortality. In select mouse strains with underlying liver disease, meaningful comparisons can be made even with ligation of a single lobe of the liver, which can reduce animal losses and expenses. Here, we describe partial bile duct ligation (pBDL) in the mouse, in which only the left hepatic bile duct is ligated, causing biliary obstruction in the left lobe but not the remaining lobes. With careful microsurgical technique, pBDL experiments can be cost-effective, since the unligated lobe serves as an internal control to the ligated lobes, when subjected to the same conditions in the same animal. Unlike cBDL, a separate sham-operated control group is not necessary. pBDL is highly useful to directly compare localized versus systemic effects of cholestasis and other retained bile components. pBDL can also be repurposed as a novel method to investigate mechanisms related to medications and cell migration.
在啮齿动物中，胆总管完全结扎（cBDL）是一种用于研究阻塞性胆汁淤积和胆管增殖的成熟外科技术。然而，长期实验会导致发病率和死亡率增加。在某些患有潜在肝脏疾病的小鼠品系中，即使只结扎一叶肝脏也能进行有意义的比较，这可以减少动物损失和费用。在此，我们描述了小鼠部分胆管结扎（pBDL），即仅结扎左肝胆管，导致左叶胆管阻塞，但其余叶胆管未阻塞。采用精细的显微外科技术，pBDL实验具有成本效益，因为在同一动物处于相同条件下时，未结扎叶可作为结扎叶的内部对照。与cBDL不同，无需单独设置假手术对照组。pBDL对于直接比较胆汁淤积和其他潴留胆汁成分的局部效应与全身效应非常有用。pBDL还可作为一种新方法用于研究与药物和细胞迁移相关的机制。
