Graduate School of Agriculture, Tamagawa University, Machida, Tokyo, 194-8610, Japan.
Sci Rep. 2022 Dec 5;12(1):20946. doi: 10.1038/s41598-022-25656-7.
To compare the behavioral roles of biogenic amines in the males of primitive and advanced eusocial bees, we determined the levels of dopamine- and octopamine-related substances in the brain, and the behavioral effects of these monoamines by drug injection in the primitive eusocial bumble bee, Bombus ignitus. The levels of dopamine and its precursors in the brain peaked at the late pupal stage, but the dopamine peak extended to adult emergence. The tyramine and octopamine levels increased from the mid-pupal to adult stages. The locomotor and flight activities, and light preference increased with age. Injection of octopamine and its receptor antagonist had significant effects on the locomotor and flight activities, whereas dopamine injection did not, indicating that these activities can be regulated by the octopaminergic system. We also determined the dynamics of dopamine-related substances in honey bee (Apis mellifera) drones. The changes in the dopamine level in the brains of honey bee drones exhibited two peaks from the pupal to adult stages, whereas the bumble bee males had only one peak. These are consistent with the behavioral functions of dopamine in honey bee drones and ineffectiveness of dopamine injection at the adult stage in bumble bee males.
为了比较生物胺在原始和高级真社会性蜜蜂雄性中的行为作用，我们测定了大脑中多巴胺和章鱼胺相关物质的水平，并通过在原始真社会性熊蜂 Bombus ignitus 中注射这些单胺来观察其行为效应。大脑中多巴胺及其前体的水平在蛹晚期达到峰值，但多巴胺峰值延伸到成虫出现。色胺和章鱼胺的水平从蛹中期增加到成虫阶段。运动和飞行活动以及对光的偏好随年龄增长而增加。注射章鱼胺及其受体拮抗剂对运动和飞行活动有显著影响，而多巴胺注射则没有，表明这些活动可以通过章鱼胺能系统进行调节。我们还测定了蜜蜂蜜蜂（Apis mellifera）雄蜂中与多巴胺相关的物质的动态变化。从蛹到成虫阶段，蜜蜂蜜蜂雄蜂大脑中的多巴胺水平显示出两个峰值，而熊蜂雄蜂只有一个峰值。这与多巴胺在蜜蜂蜜蜂雄蜂中的行为功能以及多巴胺在熊蜂雄蜂成虫阶段注射无效相一致。
