Zhou Pei-Na, Dang Jing-Jie, Shao Yong-Fang, Shi Zun-Rui, Zhang Lin, Liu Chan-Chan, Wu Qi-Nan
School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China Collaborative Innovation Center of Chinese Medicinal Resources Industrialization Nanjing 210023, China.
School of Pharmacy, Nanjing University of Chinese Medicine Nanjing 210023, China Collaborative Innovation Center of Chinese Medicinal Resources Industrialization Nanjing 210023, China National and Local Collaborative Engineering Center of Chinese Medicinal Resources Industrialization and Formulae Innovative Medicine Nanjing 210023, China.
Zhongguo Zhong Yao Za Zhi. 2022 Nov;47(21):5838-5848. doi: 10.19540/j.cnki.cjcmm.20220810.101.
Hd-Zip, a unique transcription factor in plant kingdom, influences the growth, development, and secondary metabolism of plants. Hd-zip Ⅳ is thought to play an important role in trichome development of Schizonepeta tenuifolia. This study aims to explore the functions of StHD1 and StHD8 in Hd-zip Ⅳ subfamily in peltate glandular trichome development. To be specific, the expression patterns of the two genes and interaction between the proteins encoded by them were analyzed based on transcriptome sequencing and two-hybrid screening. The subcellular localization was performed and functions of the genes were verified in tobacco and S. tenuifolia. The results showed that StHD1 and StHD8 had high similarity to HD-Zip Ⅳ proteins of other plants and they all had the characteristic conserved domains of HD-Zip Ⅳ subfamily. They were located in the nucleus. The two genes mainly expressed in young tissues and spikes, and StHD1 and StHD8 proteins interacted with each other. The density and length of glandular trichomes increased significantly in tobacco plants with the overexpression of StHD1 and StHD8. Inhibiting the expression of StHD1 and StHD8 by VIGS(virus-induced gene silencing) in S. tenuifolia resulted in the reduction in the density of peltate glandular trichomes, the expression of key genes related to mono-terpene synthesis, and the relative content of limonene and pulegone, the main components of monoterpene. These results suggested that StHD1 and StHD8 of S. tenuifolia formed a complex to regulate glandular trichomes and affect the biosynthesis of monoterpenes.
Hd-Zip是植物界中一种独特的转录因子，影响植物的生长、发育和次生代谢。Hd-zip Ⅳ被认为在荆芥的腺毛发育中起重要作用。本研究旨在探讨Hd-zip Ⅳ亚家族中的StHD1和StHD8在盾状腺毛发育中的功能。具体而言，基于转录组测序和双杂交筛选分析了这两个基因的表达模式及其编码蛋白之间的相互作用。进行了亚细胞定位，并在烟草和荆芥中验证了基因的功能。结果表明，StHD1和StHD8与其他植物的HD-Zip Ⅳ蛋白具有高度相似性，它们都具有HD-Zip Ⅳ亚家族的特征性保守结构域。它们位于细胞核中。这两个基因主要在幼嫩组织和穗中表达，且StHD1和StHD8蛋白相互作用。在过表达StHD1和StHD8的烟草植株中，腺毛的密度和长度显著增加。通过病毒诱导基因沉默（VIGS）抑制荆芥中StHD1和StHD8的表达，导致盾状腺毛密度降低、与单萜合成相关的关键基因表达降低以及单萜主要成分柠檬烯和胡薄荷酮的相对含量降低。这些结果表明，荆芥的StHD1和StHD8形成复合物来调节腺毛并影响单萜的生物合成。
