Qu Wen-Jia, Su Jia-Ming, Xu Wen-Juan, Li Chun-Shuai, Yang Lin-Lin, Zhang Shu-Yan, Wang Xuan, Cheng Shui-Qing, Wen Jia, Li Xiang-Ri
Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica, Centre of Traditional Chinese Medicine Processing Research, Beijing University of Chinese Medicine Beijing 102488, China Beijing Xicheng District Market Supervision Administration Beijing 100032, China.
Beijing Key Laboratory for Quality Evaluation of Chinese Materia Medica, Centre of Traditional Chinese Medicine Processing Research, Beijing University of Chinese Medicine Beijing 102488, China.
Zhongguo Zhong Yao Za Zhi. 2022 Nov;47(21):5855-5862. doi: 10.19540/j.cnki.cjcmm.20220627.201.
To comprehensively evaluate the quality of commercial Ginseng Radix et Rhizoma Rubra, 43 batches of commercial Ginseng Radix et Rhizoma Rubra were collected to determine the content of nine ginsenosides Rg_1, Re, Rb_1, Rk_3, Rh_4, 20(S)-Rg_3, 20(R)-Rg_3, Rk_1, and Rg_5 by high performance liquid chromatography(HPLC). The quality of the commercial Ginseng Radix et Rhizoma Rubra was evaluated by correlation analysis, principal component analysis, factor analysis, analysis of variance(ANOVA), and cluster heatmap analysis. The content determination indicated that the content of common ginsenosides in commercial Ginseng Radix et Rhizoma Rubra were higher while that of rare ginsenosides were lower. Multivariate statistical analysis revealed that ginsenosides Rg_1 and Rb_1 were significantly positively correlated with rare ginsenosides, and Rg_1, Rb_1 and rare ginsenosides played an important role in evaluating the quality of commercial Ginseng Radix et Rhizoma Rubra. In combination with the processing principle and current quality situation of Ginseng Radix et Rhizoma Rubra, it is recommended to improve the content limit of Rb_1 in the existing quality standards.
为全面评价市售红参的质量，收集43批次市售红参，采用高效液相色谱法（HPLC）测定9种人参皂苷Rg_1、Re、Rb_1、Rk_3、Rh_4、20(S)-Rg_3、20(R)-Rg_3、Rk_1和Rg_5的含量。通过相关性分析、主成分分析、因子分析、方差分析（ANOVA）和聚类热图分析对市售红参的质量进行评价。含量测定结果表明，市售红参中常见人参皂苷含量较高，而稀有皂苷含量较低。多变量统计分析表明，人参皂苷Rg_1和Rb_1与稀有皂苷呈显著正相关，Rg_1、Rb_1和稀有皂苷在评价市售红参质量中起重要作用。结合红参的炮制原理和当前质量状况，建议提高现行质量标准中Rb_1的含量限度。
