Hu Ping, Luo Guo-An, Wang Qing, Zhao Zhong-Zhen, Wang Wan, Jiang Zhi-Hong
East China University of Science and Technology, Shanghai, 200237, China.
Arch Pharm Res. 2009 May;32(5):667-76. doi: 10.1007/s12272-009-1504-7. Epub 2009 May 27.
This study systematically investigated the retention behavior of seven neutral ginsenosides Rg(1), Re, Rf, Rb(1), Rb(2), Rc, Rd, and an acidic ginsenoside R(0), the major pharmacologically active components of Radix Ginseng with RP-HPLC. The effects of solvent, pH value, ionic strength of the mobile phase, and column temperature were investigated using an octadecylsiloxane-bonded silica gel column. Based on the ginsenosides' retention characteristics, the concentration of acetonitrile and the gradient of the mobile phase needed to maintain the baseline separation of the major neutral ginsenosides in Radix Ginseng were theoretically predicted. Furthermore, the ionic strength of mobile-phase necessary to achieve good resolution of the neutral ginsenosides and acidic ginsenosides was carefully investigated. According to the results of the quantitative analysis of ginsenosides in eight batches of ginseng samples from different sources, the developed HPLC technique may be a valuable tool for the quality assessment of Radix Ginseng.
本研究采用反相高效液相色谱法（RP-HPLC）系统地研究了七种中性人参皂苷Rg(1)、Re、Rf、Rb(1)、Rb(2)、Rc、Rd以及一种酸性人参皂苷R(0)的保留行为，这些成分是人参的主要药理活性成分。使用十八烷基硅氧烷键合硅胶柱研究了溶剂、pH值、流动相离子强度和柱温的影响。基于人参皂苷的保留特性，理论预测了保持人参中主要中性人参皂苷基线分离所需的乙腈浓度和流动相梯度。此外，还仔细研究了实现中性人参皂苷和酸性人参皂苷良好分离所需的流动相离子强度。根据对八批不同来源人参样品中人参皂苷的定量分析结果，所建立的HPLC技术可能是人参质量评估的一种有价值的工具。
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