Zhang Yan-Kun, Ke Huai-Yang, Qin Yong-Qiang, Ju Han-Ye, Chen Yu-Mei, Lin Fang, Zhang Ji-Liang, Diao Xiao-Ping
Ministry of Education Key Laboratory for Ecology of Tropical Islands, Hainan Normal University, Haikou, 571158, China; College of Life Science Hainan Normal University, Haikou, Hainan, 571158, China.
College of Ecology and Environment Hainan University, Haikou, Hainan, 570228, China.
Environ Pollut. 2023 Jan 15;317:120792. doi: 10.1016/j.envpol.2022.120792. Epub 2022 Dec 3.
Benzophenone-3 (BP-3) often used as a UV filter in various products and an endocrine disruptor. In this work, we exposed the clown anemonefish to 10 μg/L and 50 μg/L BP-3 for 7 and 14 days. Liver histological, biochemical analysis, and transcriptome sequencing were used to explore the mechanism of the lipid metabolism disorder in the liver of three-month-old clown anemonefish treated with BP-3. The histological and biochemical analysis showed that BP-3 induces morphological changes and lipid droplet accumulation, and the lipid content, lipase, and antioxidant enzyme activity were abnormal. After treatment with 10 μg/L and 50 μg/L BP-3 for 7 days, the transcriptome analysis further demonstrated that the KEGG analysis revealed that the differentially expressed genes (DEGs) were mainly associated with fat digestion and absorption, PPAR signaling pathway, circadian rhythm, and mineral absorption pathways; After 10 μg/L and 50 μg/L of BP-3 exposure for 14 days, the KEGG analysis were mainly associated with circadian rhythm, circadian rhythm-fly, protein processing in the endoplasmic reticulum, and beta-alanine metabolism pathways. Several key genes were involved in the process of liver lipid metabolism, including CD36, APoA-Ⅰ, FABP, LPL, ACS, and PEPCK. The qRT-PCR validation results showed that eight genes (CYP8B1, FABP1, LPL, MGAT, PEPCK, PER1, PSMB4, PSME2) were significantly down-regulated, and the other two genes (Fbxl3, RXR) were significantly up-regulated after 7 days of BP-3 exposure. Similarly, eleven genes (AMPK, ARNTL, Bmal1, CASP3, CYC, CYP2J, CYP2U1, GSK3A, PEPCK, RAC1, RORA) were significantly up-regulated, and the other four genes (NR1D1, PER1, PTGDS, HLF) were significantly down-regulated after 14 days of BP-3 exposure. In conclusion, our results elucidate the physiological and molecular responses to BP-3 exposure in the liver lipid metabolism of clown anemonefish, and these findings reveal that the regulation of lipid metabolism is disturbed when clown anemonefish is exposed to UV filters.
二苯甲酮 -3（BP -3）常用于各种产品中的紫外线过滤剂，也是一种内分泌干扰物。在本研究中，我们将小丑鱼暴露于10μg/L和50μg/L的BP -3中，处理7天和14天。通过肝脏组织学、生化分析以及转录组测序，探究BP -3处理对3个月大小丑鱼肝脏脂质代谢紊乱的机制。组织学和生化分析表明，BP -3诱导形态变化和脂滴积累，脂质含量、脂肪酶和抗氧化酶活性异常。用10μg/L和50μg/L的BP -3处理7天后，转录组分析进一步表明，KEGG分析显示差异表达基因（DEGs）主要与脂肪消化与吸收、PPAR信号通路、昼夜节律和矿物质吸收途径相关；用10μg/L和50μg/L的BP -3暴露14天后，KEGG分析主要与昼夜节律、昼夜节律 - 果蝇、内质网中的蛋白质加工以及β - 丙氨酸代谢途径相关。几个关键基因参与了肝脏脂质代谢过程，包括CD36、APoA -Ⅰ、FABP、LPL、ACS和PEPCK。qRT - PCR验证结果表明，BP -3暴露7天后，八个基因（CYP8B1、FABP1、LPL、MGAT、PEPCK、PER1、PSMB4、PSME2）显著下调，另外两个基因（Fbxl3、RXR）显著上调。同样，BP -3暴露14天后，十一个基因（AMPK、ARNTL、Bmal1、CASP3、CYC、CYP2J、CYP2U1、GSK3A、PEPCK、RAC1、RORA）显著上调，另外四个基因（NR1D1、PER1、PTGDS、HLF）显著下调。总之，我们的结果阐明了小丑鱼肝脏脂质代谢对BP -3暴露的生理和分子反应，这些发现揭示了小丑鱼暴露于紫外线过滤剂时脂质代谢调节受到干扰。
