Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan; Center of Advanced Technology for the Environment, Graduate School of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
Faculty of Agriculture, Ehime University, 3-5-7 Tarumi, Matsuyama, Ehime 790-8566, Japan.
Mar Pollut Bull. 2024 Apr;201:116260. doi: 10.1016/j.marpolbul.2024.116260. Epub 2024 Mar 23.
This study aimed to investigate the toxic and transcriptomic effects of the ultraviolet filter benzophenone-3 (BP-3) on Acropora tenuis and its symbiotic dinoflagellates while using acetone as a solvent. Seven-day exposure to 50 and 500 μg/L, which is higher than most BP-3 records from coastal waters, did not affect coral colour or dinoflagellate photosynthesis. Differentially expressed genes (DEGs) between seawater and solvent controls were <20 in both corals and dinoflagellates. Eleven coral DEGs were detected after treatment with 50 μg/L BP-3. Fourteen coral DEGs, including several fluorescent protein genes, were detected after treatment with 500 μg/L BP-3. In contrast, no dinoflagellate DEGs were detected in the BP-3 treatment group. These results suggest that the effects of 50-500 μg/L BP-3 on adult A. tenuis and its dinoflagellates are limited. Our experimental methods with lower acetone toxicity provide a basis for establishing standard ecotoxicity tests for corals.
本研究旨在探究紫外线过滤剂二苯甲酮-3（BP-3）对纤细盔形珊瑚及其共生虫黄藻的毒性和转录组效应，同时使用丙酮作为溶剂。在为期 7 天的暴露实验中，浓度分别为 50 和 500μg/L 的 BP-3（高于大多数沿海海域的 BP-3 记录浓度）并未影响珊瑚的颜色或虫黄藻的光合作用。在珊瑚和虫黄藻中，海水和溶剂对照组之间差异表达的基因（DEGs）均<20。在 50μg/L BP-3 处理后，检测到 11 个珊瑚 DEGs。在 500μg/L BP-3 处理后，检测到 14 个珊瑚 DEGs，包括几个荧光蛋白基因。相比之下，在 BP-3 处理组中未检测到虫黄藻 DEGs。这些结果表明，50-500μg/L BP-3 对成年纤细盔形珊瑚及其虫黄藻的影响有限。我们采用毒性较低的丙酮的实验方法，为珊瑚的标准生态毒性测试奠定了基础。
