Garcia de Carvalho Gabriel, Pacheco Mateo Rafaela, Costa E Silva Rodrigo, Maquera Huacho Patricia Milagros, de Souza Rastelli Alessandra Nara, de Oliveira Kleber Thiago, Chierici Marcantonio Rosemary Adriana, Zandim-Barcelos Daniela Leal, Palomari Spolidorio Denise Madalena
Department of Diagnosis and Surgery, School of Dentistry, São Paulo State University (UNESP), Rua Humaitá, 1680, Araraquara, SP 14801-903, Brazil.
Department of Chemistry, Federal University of São Carlos (UFSCar), São Carlos, SP 13565-905, Brazil.
Photodiagnosis Photodyn Ther. 2023 Mar;41:103219. doi: 10.1016/j.pdpdt.2022.103219. Epub 2022 Dec 5.
In our previous study, Chlorin-e6 (Ce6) demonstrated a significant reduction of microorganisms' viability against single-species biofilm related to periodontitis once irradiated by red light (660 nm). Also, higher bacteria elimination was observed under blue light (450 nm) irradiation. However, the use of blue light irradiation of Ce6 for antimicrobial administration is poorly explored. This study evaluated the effect of chlorin-e6-mediated antimicrobial photodynamic therapy (aPDT) using different wavelengths (450 or 660 nm) against multi-species biofilms related to periodontitis. Streptococcus oralis, Fusobacterium nucleatum, Porphyromonas gingivalis, and Aggregatibacter actinomycetemcomitans composed the mature biofilm developed under proper conditions for five days. aPDT was performed using different concentrations of Ce6 (100 and 200 μM), wavelengths (450 or 660 nm), and comparisons were made after qPCR assay and confocal laser scanning microscopy (CLSM) analysis. The greatest bacterial elimination was observed in the groups where Ce6 was used with blue light, for S. orallis (2.05 Log GeQ mL-1, p < 0.0001) and P. gingivalis (1.4 Log GeQ mL-1, p < 0.0001), aPDT with red light showed significant bacteria reduction only for S. orallis. aPDT with blue light demonstrated statistically higher elimination in comparison with aPDT with red light. The aPDT did not show a statistically significant effect when tested against A. actinomycetemcomitans and F. nucleatum (p=0.776 and 0.988, respectively). The aPDT using blue light showed a promising higher photobiological effect, encouraging researchers to consider it in the irradiation of Ce6 for further investigations.
在我们之前的研究中，二氢卟吩 - e6（Ce6）经红光（660nm）照射后，对与牙周炎相关的单菌种生物膜中的微生物活力有显著降低作用。此外，在蓝光（450nm）照射下观察到更高的细菌清除率。然而，关于使用Ce6蓝光照射进行抗菌给药的研究较少。本研究评估了使用不同波长（450或660nm）的二氢卟吩 - e6介导的抗菌光动力疗法（aPDT）对与牙周炎相关的多菌种生物膜的影响。口腔链球菌、具核梭杆菌、牙龈卟啉单胞菌和伴放线聚集杆菌构成了在适当条件下培养5天形成的成熟生物膜。使用不同浓度的Ce6（100和200μM）、波长（450或660nm）进行aPDT，并在qPCR检测和共聚焦激光扫描显微镜（CLSM）分析后进行比较。在Ce6与蓝光联合使用的组中观察到最大的细菌清除率，对于口腔链球菌（2.05 Log GeQ mL-1，p < 0.0001）和牙龈卟啉单胞菌（1.4 Log GeQ mL-1，p < 0.0001），红光aPDT仅对口腔链球菌显示出显著的细菌减少。与红光aPDT相比，蓝光aPDT在统计学上显示出更高的清除率。当针对伴放线聚集杆菌和具核梭杆菌进行测试时，aPDT没有显示出统计学上的显著效果（分别为p = 0.�76和0.988）。使用蓝光的aPDT显示出有前景的更高光生物学效应，鼓励研究人员在Ce6照射中考虑它以进行进一步研究。
