比较转录组分析揭示了烟粉虱在单独或联合应用钝绥螨（Zare & Gams）和苦参碱后基因表达的差异。

Comparative transcriptome analysis reveals differences in gene expression in whitefly following individual or combined applications of Akanthomyces attenuatus (Zare & Gams) and matrine.

机构信息

Key Laboratory of Bio-Pesticide Innovation and Application, Engineering Research Centre of Biological Control, South China Agricultural University, Guangzhou, 510642, China.

Engineering Research Center of Biological Control, Ministry of Education and Guangdong Province, South China Agricultural University, Guangzhou, 510642, China.

出版信息

BMC Genomics. 2022 Dec 6;23(1):808. doi: 10.1186/s12864-022-09048-9.

DOI:10.1186/s12864-022-09048-9

PMID:36474158

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9727895/

Abstract

BACKGROUND

Bemisia tabaci Gennadius (Hemiptera: Aleyrodidae) is a serious pest of crops in different regions of the world. Our recent studies on the joint application of Akanthomyces attenuatus (a pathogenic insect fungus) and matrine (a botanical insecticide) against B. tabaci have shown promising results. Using RNA sequencing (RNA-Seq), we identified differentially expressed genes involved in whitefly responses to single or mixed applications of A. attenuatus and matrine.

METHODS

In this study, we compared the transcriptome profiles of B. tabaci treated with individual and combined treatments of A. attenuatus and matrine to determine variations in gene expression among whiteflies in response to different treatments.

RESULTS

Transcriptomic data analysis showed differential expression of 71, 1194, and 51 genes in response to A. attenuatus (BtA), matrine (BtM), and A. attenuatus + matrine (BtAM) treatment, respectively. A total of 65 common differentially expressed genes (DEGs) were identified between whiteflies treated with A. attenuatus (BtA) and matrine (BtM). A comparison of DEGs across the three treatments (BtA, BtM, and BtAM) revealed two common DEGs. The results also revealed that AMPK signaling, apoptosis, and drug metabolism pathways are likely involved in whitefly defense responses against A. attenuatus and matrine infection. Furthermore, a notable suppression of general metabolism and immune response genes was observed in whiteflies treated with A. attenuatus + matrine (BtAM) compared to whiteflies treated with individual A. attenuatus (BtA) or matrine (BtM) treatments.

CONCLUSION

Dynamic changes in the number of differentially expressed genes were observed in B. tabaci subjected to different treatments (BtA, BtM, and BtAM). To the best of our knowledge, this is the first report on the molecular interactions between whitefly and individual or combined treatments of A. attenuatus and matrine. These results will further improve our knowledge of the infection mechanism and complex biochemical processes involved in the synergistic action of A. attenuatus and matrine against B. tabaci.

摘要

背景

烟粉虱（半翅目：粉虱科）是世界不同地区作物的严重害虫。我们最近对阿克曼木霉菌（一种病原性昆虫真菌）和苦参碱（一种植物性杀虫剂）联合应用防治烟粉虱的研究表明，这一联合应用具有良好的效果。采用 RNA 测序（RNA-Seq）技术，我们鉴定了烟粉虱对阿克曼木霉菌和苦参碱单一或混合应用的反应中涉及的差异表达基因。

方法

在这项研究中，我们比较了单独和联合应用阿克曼木霉菌和苦参碱处理的烟粉虱的转录组谱，以确定烟粉虱对不同处理的基因表达变化。

结果

转录组数据分析显示，阿克曼木霉菌（BtA）、苦参碱（BtM）和阿克曼木霉菌+苦参碱（BtAM）处理的烟粉虱分别有 71、1194 和 51 个基因表达差异。在阿克曼木霉菌（BtA）和苦参碱（BtM）处理的烟粉虱中，共鉴定出 65 个共同差异表达基因（DEGs）。对三种处理（BtA、BtM 和 BtAM）的 DEGs 进行比较，发现了两个共同的 DEGs。结果还表明，AMPK 信号转导、细胞凋亡和药物代谢途径可能参与了烟粉虱对阿克曼木霉菌和苦参碱感染的防御反应。此外，与单独用阿克曼木霉菌（BtA）或苦参碱（BtM）处理的烟粉虱相比，用阿克曼木霉菌+苦参碱（BtAM）处理的烟粉虱的一般代谢和免疫反应基因显著受到抑制。