眼镜蛇毒因子（CVF）诱导的大蜡螟血淋巴中的C3转化酶活性。

A cobra venom factor (CVF)-induced C3 convertase activity in the hemolymph of Galleria mellonella.

作者信息

Phipps D, Menger M, Chadwick J S, Aston W P

出版信息

Dev Comp Immunol. 1987 Winter;11(1):37-46. doi: 10.1016/0145-305x(87)90006-1.

Abstract

A cobra venom factor (CVF)-induced C3 convertase has been generated from the hemolymph of Galleria mellonella. CVF was immobilized on Sepharose 4B and treated with cell-free hemolymph obtained from either unvaccinated G. mellonella larvae or larvae immunized with formalized Pseudomonas aeruginosa. The C3-cleaving activity was detected by the ability to cleave the alpha-chain of bovine C3 in a manner analogous to the CVF-induced mammalian C3 convertase, CVF,Bb. The insect-derived C3 convertase formed at 28 degrees C but not at 37 degrees C, then once formed was active at both 28 degrees C and 37 degrees C. EDTA did not inhibit the formation and action of the insect derived C3 cleaving activity.

摘要

已从大蜡螟的血淋巴中产生了一种眼镜蛇毒因子（CVF）诱导的C3转化酶。将CVF固定在琼脂糖4B上，并用从未接种疫苗的大蜡螟幼虫或用甲醛化铜绿假单胞菌免疫的幼虫获得的无细胞血淋巴进行处理。通过以类似于CVF诱导的哺乳动物C3转化酶CVF,Bb的方式切割牛C3的α链的能力来检测C3裂解活性。昆虫来源的C3转化酶在28℃时形成，但在37℃时不形成，一旦形成，在28℃和37℃时均有活性。EDTA不抑制昆虫来源的C3裂解活性的形成和作用。