Pryce M J, Aston W P, Chadwick J S
Department of Microbiology and Immunology, Queen's University, Kingston, Ontario, Canada.
Dev Comp Immunol. 1990 Fall;14(4):369-78. doi: 10.1016/0145-305x(90)90029-e.
The injection of cane sugar factor (CSF) into Galleria mellonella larvae results in an immune response similar to that produced by a formalized Pseudomonas aeruginosa vaccine. Vaccination with CSF is followed by: an increase in the LD50 of Pseudomonas aeruginosa; an in vivo protective response to P. aeruginosa the development of which can be inhibited by cobra venom factor (CVF); an antibacterial activity in hemolymph 24 h after the injection of CSF; the development of a transferrable immune response in hemolymph of donor larvae capable of protecting recipient larvae against a lethal challenge of Pseudomonas aeruginosa; an increase in extracellular lysozyme equal to that induced by Pseudomonas vaccine; a reduction in total hemocyte count during the period of protective immunity; and the presence in hemolymph of new basic proteins, with electrophoretic mobilities and appearance times after the CSF injection, identical to those induced by the formalized vaccine. CSF was shown to be composed primarily of glucose.
将蔗糖因子（CSF）注射到黄粉虫幼虫体内会引发一种免疫反应，该反应类似于由甲醛化铜绿假单胞菌疫苗所产生的免疫反应。用CSF进行疫苗接种后会出现以下情况：铜绿假单胞菌的半数致死剂量（LD50）增加；对铜绿假单胞菌产生体内保护反应，其发展可被眼镜蛇毒因子（CVF）抑制；注射CSF后24小时血淋巴中出现抗菌活性；供体幼虫血淋巴中产生可转移的免疫反应，能够保护受体幼虫免受铜绿假单胞菌的致死性攻击；细胞外溶菌酶增加，与铜绿假单胞菌疫苗诱导的增加量相同；在保护性免疫期间总血细胞计数减少；血淋巴中出现新的碱性蛋白，其电泳迁移率和CSF注射后的出现时间与甲醛化疫苗诱导产生的相同。结果表明，CSF主要由葡萄糖组成。
