Yu Xin, Lu Jian Feng, Gao Mei Qin, Xiong Bin, Xia Wen Qian
Chin J Dent Res. 2022 Dec 8;25(4):269-275. doi: 10.3290/j.cjdr.b3628171.
To screen miRNAs that could simultaneously regulate osteo/odontogenic differentiation of multiple stem cells, including dental pulp stem cells (DPSCs), stem cells from the apical papilla (SCAPs) and periodontal ligament stem cells (PDLSCs).
Differentially expressed miRNAs analysis on three miRNA microarrays data of dental stem cells undergoing osteo/odontogenic differentiation (GSE138180, GSE154466 and GSE159508) was performed, and miR-146a-5p were identified by bioinformatic prediction, dual-luciferase reporter assay and quantitative real-time polymerase chain reaction (PCR). In addition, differentially expressed genes between miR-146a-5p overexpressed group and control group (GSE79341) were applied for KEGG pathways enrichment analysis.
MiR-146a-5p expression increased in the osteo/odontogenic differentiation of DPSCs, SCAPs and PDLSCs. Tumour necrosis factor receptor (TNFR)-associated factor 6 (TRAF6) was identified as the target gene of miR-146a-5p. Furthermore, miR-146a-5p could influence the NF-Kappa B signalling pathway.
This study suggests that miR-146a-5p could promote differentiation in multiple dental stem cells through the NF-Kappa B signalling pathway by targeting TRAF6.
筛选能够同时调节多种干细胞（包括牙髓干细胞（DPSC）、根尖乳头干细胞（SCAP）和牙周膜干细胞（PDLSC））的成骨/牙源性分化的微小RNA（miRNA）。
对成骨/牙源性分化的牙干细胞的三个miRNA微阵列数据（GSE138180、GSE154466和GSE159508）进行差异表达miRNA分析，并通过生物信息学预测、双荧光素酶报告基因检测和定量实时聚合酶链反应（PCR）鉴定miR-146a-5p。此外，将miR-146a-5p过表达组与对照组之间的差异表达基因（GSE79341）用于KEGG通路富集分析。
miR-146a-5p在DPSC、SCAP和PDLSC的成骨/牙源性分化中表达增加。肿瘤坏死因子受体（TNFR）相关因子6（TRAF6）被鉴定为miR-146a-5p的靶基因。此外，miR-146a-5p可影响核因子κB信号通路。
本研究表明，miR-146a-5p可通过靶向TRAF6，通过核因子κB信号通路促进多种牙干细胞的分化。
