He Jian, Yang Lin, Zhang Ying, Li Ranhui, Wu Jingjun, Cao Qianqian, Li Sang, Wu Xian, Duan Yunfeng, He Dongxiu, Wang Weiguo
Institute of Pharmacy and Pharmacology, University of South China, Hengyang 421000, Hunan, China.
Hunan Province Cooperative Innovation Center for Molecular Target New Drug Study, Hengyang, Hunan, China.
Anal Methods. 2022 Dec 22;15(1):8-16. doi: 10.1039/d2ay01560a.
The design of highly active nanozymes and the establishment of ultra-sensitive bioassays remain a challenge. Therefore, it is necessary to synthesize highly active nanozymes. In this work, a Pd-Pt-Ru (PPR) nanozyme was prepared by atomic coating of the bimetallic nanozyme Pd-Pt. The steady-state kinetics showed that the PPR nanozyme had excellent peroxidase-like activity. Based on this concept, the as-prepared PPR nanozyme was applied to the detection of ascorbic acid (AA) and hydrogen peroxide (HO). The linear ranges for ascorbic acid and hydrogen peroxide were 2-12 μM and 5-40 mM, respectively. The limits of detection (LOD) are 1.13 μM and 2.79 mM, respectively. Ascorbic acid was used as a typical model to assay the total antioxidant capacity (TAC) of foods and several herbs. The extract showed the highest reducing ability. The corresponding extracts were applied for the green synthesis of silver nanoparticles with a size of 167 nm. This study provides a method for the design of highly active nanozymes and the expansion of their applications.
高活性纳米酶的设计以及超灵敏生物检测方法的建立仍然是一项挑战。因此，有必要合成高活性纳米酶。在本研究中，通过对双金属纳米酶Pd-Pt进行原子包覆制备了一种Pd-Pt-Ru（PPR）纳米酶。稳态动力学表明，PPR纳米酶具有优异的类过氧化物酶活性。基于这一概念，将所制备的PPR纳米酶应用于抗坏血酸（AA）和过氧化氢（H₂O₂）的检测。抗坏血酸和过氧化氢的线性范围分别为2 - 12 μM和5 - 40 mM。检测限（LOD）分别为1.13 μM和2.79 mM。以抗坏血酸作为典型模型来测定食品和几种草药的总抗氧化能力（TAC）。提取物显示出最高的还原能力。相应的提取物被用于绿色合成尺寸为167 nm的银纳米颗粒。本研究为高活性纳米酶的设计及其应用拓展提供了一种方法。
