School of Chemistry and Chemical Engineering/Guangzhou Key Laboratory for Clean Energy and Materials/Key Laboratory for Water Quality and Conservation of the Pearl River Delta, Ministry of Education, Guangzhou University, Guangzhou, Guangdong, 510006, People's Republic of China; Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha, 410082, People's Republic of China.
Molecular Science and Biomedicine Laboratory (MBL), State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Aptamer Engineering Center of Hunan Province, Hunan Provincial Key Laboratory of Biomacromolecular Chemical Biology, Hunan University, Changsha, 410082, People's Republic of China.
Biosens Bioelectron. 2023 Sep 15;236:115416. doi: 10.1016/j.bios.2023.115416. Epub 2023 May 24.
Nanozyme-based colorimetric assays have aroused extensive attention in biosensing due to quick response, low cost and simplicity. However, their practical applications are limited by the unsatisfactory stability and catalytic activity of nanozymes in complex detection environments. Herein, using the one-pot chemical vapor deposition method, we successfully prepare a highly efficient and stable carbon supported Co-Ir nanozyme (termed as Co-Ir/C nanozyme) for the determination of total antioxidant capacity (TAC) in food samples. The Co-Ir/C nanozyme shows excellent durability under extensive pH ranges, high temperature and high salt concentration due to the protection of carbon supporter. It can be recycled by simple magnetic separation, and its catalytic activity remains stable after long-term operation and storage. Taking full advantage of the superior peroxidase-like activity of Co-Ir/C nanozyme, it is used for colorimetric detection of ascorbic acid (or known as vitamin C), an important vitamin to adjust body's normal physiological function, with results showing higher sensitivity (detection limit of 0.27 μM) than most of the recently published works. Moreover, the determination of TAC in vitamin C tablets and fruits are further achieved, which are in good agreement with those of commercial colorimetric test kits. This study helps guide the rational preparation of versatile and highly stable nanozymes, and develops a robust TAC determination platform for future food quality monitoring.
基于纳米酶的比色分析由于响应迅速、成本低、操作简单，在生物传感领域引起了广泛关注。然而，由于纳米酶在复杂检测环境中的稳定性和催化活性不理想，其实际应用受到限制。在此，我们采用一锅化学气相沉积法成功制备了一种高效稳定的碳负载 Co-Ir 纳米酶（称为 Co-Ir/C 纳米酶），用于测定食品样品中的总抗氧化能力（TAC）。由于碳载体的保护，Co-Ir/C 纳米酶在广泛的 pH 值范围、高温和高盐浓度下具有出色的耐用性。它可以通过简单的磁分离进行回收，并且经过长期操作和储存后，其催化活性保持稳定。充分利用 Co-Ir/C 纳米酶优异的过氧化物酶样活性，用于测定抗坏血酸（又称维生素 C），这是一种调节人体正常生理功能的重要维生素，其检测灵敏度（检测限为 0.27 μM）高于最近发表的大多数研究结果。此外，还进一步实现了维生素 C 片剂和水果中 TAC 的测定，与商业比色试剂盒的测定结果吻合良好。本研究有助于指导多功能和高稳定性纳米酶的合理制备，并为未来的食品质量监测开发了一种强大的 TAC 测定平台。
