Ochlich Désirée, Rademacher Franziska, Drerup Katharina Antonia, Gläser Regine, Harder Jürgen
Department of Dermatology, Kiel University, Kiel, Germany.
Exp Dermatol. 2023 Apr;32(4):555-561. doi: 10.1111/exd.14727. Epub 2022 Dec 20.
The skin microbiota is a crucial component in maintaining cutaneous barrier function. Staphylococcus epidermidis is considered as a beneficial commensal member of the cutaneous microbiota promoting skin health. However, S. epidermidis is also frequently detectable in the skin of patients with the inflammatory skin disease atopic dermatitis (AD) and some studies reported a significantly higher presence of S. epidermidis in severe AD as compared to mild AD. Therefore, this study aimed to analyse the impact of S. epidermidis on the expression of cutaneous inflammatory mediators and skin barrier molecules. Various S. epidermidis skin-derived isolates activated the proinflammatory transcription factor NF-kappaB and induced expression of AD-associated proinflammatory cytokines in human primary keratinocytes and 3D skin equivalents. Skin barrier molecules such as filaggrin were downregulated by S. epidermidis. In general, AD-derived S. epidermidis strains elicited a higher response than strains derived from the skin of healthy individuals. Taken together, our results provide further evidence that the abundance of S. epidermidis in AD may trigger the inflammatory scenario associated with this disease.
皮肤微生物群是维持皮肤屏障功能的关键组成部分。表皮葡萄球菌被认为是促进皮肤健康的皮肤微生物群中有益的共生成员。然而，在炎症性皮肤病特应性皮炎（AD）患者的皮肤中也经常能检测到表皮葡萄球菌，一些研究报告称，与轻度AD相比，严重AD患者皮肤中表皮葡萄球菌的存在显著更高。因此，本研究旨在分析表皮葡萄球菌对皮肤炎症介质和皮肤屏障分子表达的影响。各种源自皮肤的表皮葡萄球菌分离株激活了促炎转录因子NF-κB，并在人原代角质形成细胞和三维皮肤等效物中诱导了与AD相关的促炎细胞因子的表达。表皮葡萄球菌下调了如丝聚合蛋白等皮肤屏障分子。一般来说，源自AD的表皮葡萄球菌菌株比源自健康个体皮肤的菌株引发的反应更高。综上所述，我们的结果提供了进一步的证据，表明AD中表皮葡萄球菌的丰度可能引发与该疾病相关的炎症情况。
