College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China.
College of Horticulture and Plant Protection, Yangzhou University, Yangzhou 225009, China; Jiangsu Co-Innovation Center for Modern Production Technology of Grain Crops, Yangzhou University, Yangzhou 225009, China.
Virus Res. 2023 Jan 15;324:199019. doi: 10.1016/j.virusres.2022.199019. Epub 2022 Dec 8.
Rice stripe virus (RSV) causes enormous losses in rice production and is transmitted by the small brown planthopper, Laodelphax striatellus, in a persistent-propagative manner. RSV accumulation within the gut lumen of the vector is indispensable for the successful transmission to rice and insects. In this study, we obtained a 1464 bp full-length cDNA of a voltage-dependent anion channel 2 from L. striatellus (LsVDAC2), which encodes a 283 amino acid protein. RSV infection increased the expression of LsVDAC2 in the midguts and ovaries of L. striatellus by 260% and 228%, respectively. Silencing of LsVDAC2 resulted in a 88% reduction of RSV loads at 24 h after RNAi, indicating that LsVDAC2 facilitates RSV accumulation in the vector. Yeast two-hybrid and GST pulldown assays demonstrated that LsVDAC2 interacted with RSV RNA-dependent RNA polymerase, RdRp. Furthermore, experiments in vivo and in vitro showed that LsVDAC2 induced the apoptotic response in RSV-infected insects and tissues. Silencing of LsVDAC2 via RNAi significantly reduced the expression of genes for apoptosis-related caspases 1a and 1c by 62% and 78%, respectively, in RSV-infected vectors. Whether LsVDAC2-induced RSV accumulation is related to RSV RdRp and LsVDAC2-induced cell apoptosis deserves further investigation.
水稻条纹病毒（RSV）可导致水稻严重减产，其通过迁飞性昆虫褐飞虱以持久增殖的方式传播。RSV 在介体昆虫肠道中的积累对成功传播至水稻和昆虫是不可或缺的。在本研究中，我们从褐飞虱中获得了一个全长为 1464bp 的电压依赖性阴离子通道 2 的 cDNA（LsVDAC2），该基因编码一个 283 个氨基酸的蛋白。RSV 感染分别使 L. striatellus 中肠和卵巢中 LsVDAC2 的表达增加了 260%和 228%。RNAi 后 24 小时，LsVDAC2 沉默导致 RSV 载量降低了 88%，表明 LsVDAC2 有助于 RSV 在介体中的积累。酵母双杂交和 GST 下拉实验表明，LsVDAC2 与 RSV RNA 依赖性 RNA 聚合酶 RdRp 相互作用。此外，体内和体外实验表明，LsVDAC2 诱导 RSV 感染昆虫和组织发生细胞凋亡。通过 RNAi 沉默 LsVDAC2 可使 RSV 感染的介体中与细胞凋亡相关的半胱氨酸天冬氨酸蛋白酶 1a 和 1c 的表达分别降低 62%和 78%。LsVDAC2 诱导 RSV 积累是否与 RSV RdRp 和 LsVDAC2 诱导的细胞凋亡有关，值得进一步研究。
