State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
State Key Laboratory for Managing Biotic and Chemical Threats to the Quality and Safety of Agro-Products, Key Laboratory of Biotechnology in Plant Protection of Ministry of Agriculture and Zhejiang Province, Institute of Plant Virology, Ningbo University, Ningbo 315211, China.
J Proteomics. 2021 May 15;239:104184. doi: 10.1016/j.jprot.2021.104184. Epub 2021 Mar 9.
Persistent plant viruses multiply and circulate inside insect vectors following the route of midgut-hemolymph-salivary gland. Currently, how viruses interact with insect vectors after they are released into hemolymph is not entirely clear. In this study, we found that the hemolymph and fat body (HF) contained the highest Rice stripe virus (RSV) levels. Proteomic analysis on RSV-free and RSV-infected HF identified 156 differentially expressed proteins (DEPs), with the majority of them participating in metabolism, transportation, and detoxification. The RNA binding protein esf2 was the most downregulated protein. Knocking down the expression of esf2 did not influence the RSV burden, but caused the lethal effect to L. striatellus. In contrast, the mRNA decay protein ZFP36L1 was 69% more abundant upon RSV infection, and suppression of ZFP36L1 significantly increased the RSV burden. Our results reveal the potential role of ZFP36L1 in restricting the viral proliferation, and provide valuable clues for unravelling the interaction between RSV and L. striatellus in HF. SIGNIFICANCE: More than 76% of plant viruses are transmitted by insect vectors. For persistent propagative transmission, plant viruses multiply and circulate inside insects following the route of midgut-hemolymph-salivary gland. However, how viruses interact with vector insects after they are released into hemolymph is not entirely clear. Our study investigated the influence of rice stripe virus (RSV) on insect hemolymph and fat body by iTRAQ labeling method. Among the 156 differentially expressed proteins (DEPs) identified, two proteins associated with mRNA metabolism were selected for function analysis. We found that the mRNA decay activator protein ZFP36L1 influenced the RSV proliferation, and RNA binding protein esf2 caused the lethal effect to L. striatellus. Our results provide valuable clues for unveiling the interaction between RSV and L. striatellus, and might be useful in pest management.
持久型植物病毒在进入昆虫血腔后，通过中肠-血腔-唾液腺的途径进行复制和循环。目前，病毒进入血腔后如何与昆虫媒介相互作用尚不完全清楚。在本研究中，我们发现血腔和脂肪体（HF）中含有最高水平的水稻条纹病毒（RSV）。对无 RSV 和 RSV 感染的 HF 进行蛋白质组学分析，共鉴定出 156 个差异表达蛋白（DEPs），其中大多数参与代谢、运输和解毒。RNA 结合蛋白 esf2 是下调最明显的蛋白。敲低 esf2 的表达并不影响 RSV 的负担，但会导致稻绿蝽致死。相比之下，在 RSV 感染时，mRNA 降解蛋白 ZFP36L1 的丰度增加了 69%，而抑制 ZFP36L1 显著增加了 RSV 的负担。我们的研究结果揭示了 ZFP36L1 在限制病毒增殖方面的潜在作用，并为揭示 RSV 和稻绿蝽在 HF 中的相互作用提供了有价值的线索。意义：超过 76%的植物病毒是由昆虫媒介传播的。对于持久性增殖传播，植物病毒在进入昆虫血腔后，通过中肠-血腔-唾液腺的途径进行复制和循环。然而，病毒进入血腔后如何与媒介昆虫相互作用尚不完全清楚。本研究通过 iTRAQ 标记法研究了水稻条纹病毒（RSV）对昆虫血腔和脂肪体的影响。在鉴定的 156 个差异表达蛋白（DEPs）中，选择了两个与 mRNA 代谢相关的蛋白进行功能分析。我们发现，mRNA 降解激活蛋白 ZFP36L1 影响 RSV 的增殖，而 RNA 结合蛋白 esf2 导致稻绿蝽致死。我们的研究结果为揭示 RSV 和稻绿蝽的相互作用提供了有价值的线索，并可能有助于害虫管理。
