Serradell Antonio, Montero Daniel, Fernández-Montero Álvaro, Terova Genciana, Makol Alex, Valdenegro Victoria, Acosta Félix, Izquierdo María Soledad, Torrecillas Silvia
Grupo de Investigación en Acuicultura (GIA), IU-ECOAQUA, Universidad de las Palmas de Gran Canaria, 35200 Las Palmas, Spain.
Department of Pathobiology, School of Veterinary Medicine, University of Pennsylvania, Philadelphia, PA 19104, USA.
Animals (Basel). 2022 Nov 28;12(23):3332. doi: 10.3390/ani12233332.
The aim of the present study is to evaluate the potential of two functional additives as gill endogenous antioxidant capacity boosters in European sea-bass juveniles fed low-FM/FO diets when challenged against physical and biological stressors. For that purpose, two isoenergetic and isonitrogenous diets with low FM (10%) and FO (6%) contents were supplemented with 5000 ppm plant-derived galactomannan-oligosaccharides (GMOS) or 200 ppm of a mixture of garlic and labiate plant essential oils (PHYTO). A control diet was void from supplementation. Fish were fed the experimental diet for nine weeks and subjected to a confinement stress challenge (C challenge) or a confinement stress challenge combined with an exposure to the pathogen (CI challenge). Both GMOS and PHYTO diets attenuated fish stress response, inducing lower circulating plasma cortisol and down-regulating and relative gene-expression levels in the gill. This attenuated stress response was associated with a minor energetic metabolism response in relation to the down-regulation of and gene expression.
本研究的目的是评估两种功能性添加剂在欧洲鲈鱼幼鱼食用低鱼粉/鱼油日粮并受到物理和生物应激源挑战时，作为鳃内源性抗氧化能力增强剂的潜力。为此，两种低鱼粉（10%）和低鱼油（6%）含量的等能量、等氮日粮分别添加了5000 ppm植物源半乳甘露寡糖（GMOS）或200 ppm大蒜和唇形科植物精油混合物（PHYTO）。对照日粮不添加任何物质。给鱼投喂实验日粮9周，然后使其遭受禁闭应激挑战（C挑战）或禁闭应激挑战并暴露于病原体（CI挑战）。GMOS日粮和PHYTO日粮均减轻了鱼的应激反应，导致循环血浆皮质醇水平降低，并下调鳃中相关基因的表达水平。这种减轻的应激反应与能量代谢反应减弱有关，这与相关基因表达的下调有关。
