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硫转化酶的提取、纯化、表征及其在畜牧业废水中的应用。

Extraction, Purification, Characterization and Application in Livestock Wastewater of S Sulfur Convertase.

机构信息

College of Animal Science and Technology, Jilin Agricultural University, Changchun 130000, China.

College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028000, China.

出版信息

Int J Environ Res Public Health. 2022 Dec 6;19(23):16368. doi: 10.3390/ijerph192316368.

Abstract

Sulfide is a toxic pollutant in the farming environment. Microbial removal of sulfide always faces various biochemical challenges, and the application of enzymes for agricultural environmental remediation has promising prospects. In this study, a strain of Cellulosimicrobium sp. was isolated: numbered strain L1. Strain L1 can transform S, extracellular enzymes play a major role in this process. Next, the extracellular enzyme was purified, and the molecular weight of the purified sulfur convertase was about 70 kDa. The sulfur convertase is an oxidase with thermal and storage stability, and the inhibitor and organic solvent have little effect on its activity. In livestock wastewater, the sulfur convertase can completely remove S. In summary, this study developed a sulfur convertase and provides a basis for the application in environmental remediation.

摘要

硫化物是农业环境中的一种有毒污染物。微生物去除硫化物总是面临各种生化挑战,而酶在农业环境修复中的应用具有广阔的前景。在本研究中,分离到一株纤维素分解菌:编号为菌株 L1。菌株 L1 可以转化 S,该过程中外源酶起主要作用。接下来,对胞外酶进行了纯化,纯化的硫转化酶的分子量约为 70 kDa。硫转化酶是一种具有热稳定性和储存稳定性的氧化酶,抑制剂和有机溶剂对其活性影响较小。在牲畜废水中,硫转化酶可以完全去除 S。综上所述,本研究开发了一种硫转化酶,为其在环境修复中的应用提供了依据。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9139/9740322/8d28554148b6/ijerph-19-16368-g001.jpg

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