State Key Laboratory of Urban Water Resource and Environment, School of Environment, Harbin Institute of Technology, Harbin, Heilongjiang Province, 150090, China.
Department of Geoecology and Natural Resource Management, Saint Petersburg State University, Institute of Earth Science, Russia.
Environ Res. 2022 Oct;213:113674. doi: 10.1016/j.envres.2022.113674. Epub 2022 Jun 11.
Pseudomonas sp. C27 can achieve the conversion of toxic sulfide to economical elemental sulfur (S) with various electron acceptors. In this study the distribution pattern of S produced by C27 in denitrifying sulfide removal (DSR) process was explored. The SEM observation identified that the particle size of the biogenic S was at micron level. Strikingly, a novel distribution pattern of S was revealed that the produced S was not directly secreted extracellularly, but be stored temporarily in the cell interior. Pyrolysis at 65 °C for 20 min were recommended prior to S recovery, which could maximize the separation of extracellular polymeric substances (EPS) from C27. Furthermore, the effects of N/S molar ratio, initial sulfide concentration, and micro-oxygen condition were investigated to improve the production of S by C27. The highest S production was obtained at S/N of 3 and anaerobic condition seemed to favor the S production by C27. This study would provide a theoretical support for highly efficient sulfide removal as well as S recovery in sulfide-laden wastewater treatment.
铜绿假单胞菌 C27 可以利用各种电子受体将有毒的硫化物转化为经济的元素硫 (S)。在这项研究中，探索了 C27 在反硝化脱硫（DSR）过程中产生的 S 的分布模式。扫描电子显微镜观察确定了生物 S 的粒径在微米级。引人注目的是，揭示了一种新的 S 分布模式，即产生的 S 不是直接分泌到细胞外，而是暂时储存在细胞内部。建议在回收 S 之前，在 65°C 下进行 20 分钟的热解，这可以最大程度地将胞外聚合物 (EPS) 与 C27 分离。此外，还研究了 N/S 摩尔比、初始硫化物浓度和微氧条件对 C27 生产 S 的影响。在 S/N 为 3 和厌氧条件下，S 的产量最高，似乎有利于 C27 生产 S。本研究为含硫废水处理中高效脱硫和 S 回收提供了理论支持。
