Genética del Desarrollo de Plantas, Misión Biológica de Galicia-CSIC, P.O. Box 28, 36080 Pontevedra, Spain.
Centro de Investigación en Biotecnología Agroalimentaria (CIAIMBITAL), Universidad de Almería, 04120 Almería, Spain.
Int J Mol Sci. 2022 Nov 26;23(23):14783. doi: 10.3390/ijms232314783.
The tropical common bean ( L.) is an obligatory short-day plant that requires relaxation of the photoperiod to induce flowering. Similar to other crops, photoperiod-induced floral initiation depends on the differentiation and maintenance of meristems. In this study, the global changes in transcript expression profiles were analyzed in two meristematic tissues corresponding to the vegetative and inflorescence meristems of two genotypes with different sensitivities to photoperiods. A total of 3396 differentially expressed genes (DEGs) were identified, and 1271 and 1533 were found to be up-regulated and down-regulated, respectively, whereas 592 genes showed discordant expression patterns between both genotypes. homologues of DEGs were identified, and most of them were not previously involved in floral transition, suggesting an evolutionary divergence of the transcriptional regulatory networks of the flowering process of both species. However, some genes belonging to the photoperiod and flower development pathways with evolutionarily conserved transcriptional profiles have been found. In addition, the flower meristem identity genes and , as well as , were identified as markers to distinguish between the vegetative and reproductive stages. Our data also indicated that the down-regulation of the photoperiodic genes seems to be directly associated with promoting floral transition under inductive short-day lengths. These findings provide valuable insight into the molecular factors that underlie meristematic development and contribute to understanding the photoperiod adaptation in the common bean.
热带菜豆（L.）是一种必需的短日照植物，需要光周期的松弛来诱导开花。与其他作物一样，光周期诱导的花起始取决于分生组织的分化和维持。在这项研究中，分析了两个具有不同光周期敏感性的基因型的两个分生组织组织（对应营养和花序分生组织）中转录表达谱的全局变化。鉴定出 3396 个差异表达基因（DEG），分别有 1271 个和 1533 个基因上调和下调，而 592 个基因在两种基因型之间表现出不一致的表达模式。鉴定出 DEG 的同源物，其中大多数以前未参与花转变，表明两种物种开花过程的转录调控网络发生了进化分歧。然而，发现了一些属于光周期和花发育途径的基因，它们具有进化保守的转录谱。此外，鉴定出花分生组织身份基因 和 ，以及 作为区分营养和生殖阶段的标记。我们的数据还表明，光周期基因的下调似乎与在诱导短日长度下促进花转变直接相关。这些发现为分生组织发育的分子基础提供了有价值的见解，并有助于理解普通菜豆对光周期的适应。
