College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China.
Food Crop Research Institute, Hubei Academy of Agriculture Sciences, Wuhan 430064, China.
Int J Mol Sci. 2022 Nov 30;23(23):15042. doi: 10.3390/ijms232315042.
is a biotrophic basidiomycete fungus that causes rice kernel smut, one of the most significant diseases in hybrid rice-growing areas worldwide. Little is known about the pathogenic mechanisms and functions of effectors in . Here, we performed functional studies of the effectors in and found that, of six putative effectors tested, only ThSCSP_14 caused the cell death phenotype in epidermal cells of leaves. ThSCSP_14 was upregulated early on during the infection process, and the encoded protein was secreted. The predicted signal peptide (SP) of ThSCSP_14 was required for its ability to induce the necrosis phenotype. Furthermore, the ability of ThSCSP_14 to trigger cell death in depended on suppressing the G2 allele of Skp1 (SGT1), required for Mla12 resistance (RAR1), heat-shock protein 90 (HSP90), and somatic embryogenesis receptor-like kinase (SERK3). It is important to note that ThSCSP_14 induced a plant defense response in leaves. Hence, these results demonstrate that ThSCSP_14 is a possible effector that plays an essential role in -host interactions.
是一种生物亲和担子菌真菌，可引起稻粒黑粉病，这是全球杂交水稻种植区最严重的疾病之一。目前对该真菌中的致病机制和效应子功能知之甚少。在这里，我们对 中的效应子进行了功能研究，发现在所测试的六个假定效应子中，只有 ThSCSP_14 可引起 叶片表皮细胞的细胞死亡表型。ThSCSP_14 在感染过程的早期上调表达，并且其编码的蛋白被分泌。ThSCSP_14 的预测信号肽（SP）对于其诱导坏死表型的能力是必需的。此外，ThSCSP_14 在 中触发细胞死亡的能力取决于抑制 Skp1（SGT1）的 G2 等位基因，Skp1（SGT1）是 Mla12 抗性（RAR1）、热休克蛋白 90（HSP90）和体细胞胚胎发生受体样激酶（SERK3）所必需的。值得注意的是，ThSCSP_14 在 叶片中诱导了植物防御反应。因此，这些结果表明 ThSCSP_14 是一种可能的效应子，在 与宿主的相互作用中发挥着重要作用。
