Gronemeyer U, Pülhorn G, Müller-Ruchholtz W
Albrecht Von Graefes Arch Klin Exp Ophthalmol. 1978 Nov 30;208(4):247-62. doi: 10.1007/BF00419380.
Histological studies of interlamellar keratoplasty in different inbred strains of rats (CAP, LEW) without additional sensitization--i.e. first-set reactions--are described. The corneas of 65 eyes, after allogeneic or syngeneic grafting, were examined from the 3rd to the 90th day--every 2nd day at the beginning of the reaction and later every 5th day. After syngeneic grafting (LEW leads to LEW) a non-specific healing-reaction (only slight vascularization of the graft bed, edema, granulocytic infiltration) reached its climax on the 6th day and subsided by the 10th day. After allogeneic grafting (CAP leads to LEW; RtH-1-incompatible) the non-specific-healing reaction progressed into a second phase, namely the specific reaction: increasing infiltration of the host cornea and the graft with small lymphocytes, blast cells and macrophages, directly followed by severe vascularization, reaching its climax about the 14th day. A third, phagocytic phase succeeded the infiltration leading to elimination of the donor cells, but leaving the donor stroma undamaged. All these alterations had almost completely disappeared after 35 days. Thus, the corneal allograft reaction is discussed as a typical immunological reaction leading to the destruction of transplantation-antigen-bearing cells and permitting observation of the different reaction phases more clearly than in most other tissues.
本文描述了在不同近交系大鼠（CAP、LEW）中进行层间角膜移植的组织学研究，且无额外致敏——即首次移植反应。对65只眼睛在同种异体或同基因移植后的角膜进行了检查，时间从第3天至第90天——反应开始时每2天检查一次，之后每5天检查一次。同基因移植（LEW到LEW）后，非特异性愈合反应（仅移植床轻微血管化、水肿、粒细胞浸润）在第6天达到高峰，并在第10天消退。同种异体移植（CAP到LEW；RtH-1不相容）后，非特异性愈合反应进入第二阶段，即特异性反应：宿主角膜和移植物中出现小淋巴细胞、母细胞和巨噬细胞的浸润增加，随后直接出现严重血管化，在第14天左右达到高峰。第三阶段是吞噬阶段，继浸润之后，导致供体细胞被清除，但供体基质未受损。35天后，所有这些改变几乎完全消失。因此，角膜同种异体移植反应被视为一种典型的免疫反应，导致携带移植抗原的细胞被破坏，并且比大多数其他组织更能清晰地观察到不同的反应阶段。
