Jakobsen Nadia, Goecke Nicole Bakkegård, Pedersen Ken Steen
Department of Veterinary and Animal Sciences, Faculty of Health and Medical Sciences, University of Copenhagen, 1870, Frederiksberg C, Denmark.
Porcine Health Manag. 2022 Dec 12;8(1):49. doi: 10.1186/s40813-022-00292-9.
Post weaning diarrhoea is expected to become an increasing problem in pig herds following the outphasing of medicinal Zinc Oxide. Currently, no equally effective substitute has been found and an increase in metaphylactic batch medication with antibiotics is expected. However, prudent use of antibiotics is needed to mitigate antibiotic resistance development and one option could be pre-treatment diagnostics. Employing a point-of-care test in a herd could provide fast diagnostics and help guide antibiotic treatment. Hence, the aim of this study was to evaluate the diagnostic performance of a commercially available point-of-care test for enterotoxigenic Escherichia coli (ETEC) F4, ETEC F18 and rotavirus in weaned pigs.
In total 115 diarrheic samples from two conventional herds were included in the evaluation of the Rainbow Piglet Scours test, which was compared to microbiological PCR analyses. The comparison yielded a diagnostic sensitivity, diagnostic specificity, positive and negative predictive value of 0.28, 0.99, 0.92 and 0.70 for ETEC F4, 0.40, 0.92, 0.91 and 0.45 for ETEC F18 and 0.67, 0.88, 0.91 and 0.61 for rotavirus.
The point-of-care test yielded a low diagnostic sensitivity and a high diagnostic specificity for ETEC F4, ETEC F18 and rotavirus. Due to the high level of false negatives, the test cannot be recommended for individual diagnostics on pig-level.
随着药用氧化锌的淘汰，断奶后腹泻预计将成为猪群中日益严重的问题。目前，尚未找到同样有效的替代品，预计抗生素的预防性批量用药将会增加。然而，需要谨慎使用抗生素以减缓抗生素耐药性的发展，一种选择可能是进行预处理诊断。在猪群中采用即时检测可以提供快速诊断，并有助于指导抗生素治疗。因此，本研究的目的是评估一种市售即时检测对断奶仔猪产肠毒素大肠杆菌（ETEC）F4、ETEC F18和轮状病毒的诊断性能。
在对Rainbow Piglet Scours检测的评估中，总共纳入了来自两个传统猪群的115份腹泻样本，并与微生物PCR分析进行了比较。对于ETEC F4，比较得出的诊断敏感性、诊断特异性、阳性预测值和阴性预测值分别为0.28、0.99、0.92和0.70；对于ETEC F18，分别为0.40、0.92、0.91和0.45；对于轮状病毒，分别为0.67、0.88、0.91和0.61。
该即时检测对ETEC F4、ETEC F18和轮状病毒的诊断敏感性较低，诊断特异性较高。由于假阴性率较高，不建议在猪个体水平上使用该检测进行诊断。
