Van Breda Lechelle K, Dhungyel Om P, Ginn Andrew N, Iredell Jonathan R, Ward Michael P
The University of Sydney, Faculty of Veterinary Science, Camden, Australia.
Centre for Infectious Diseases and Microbiology, The Westmead Millennium Institute for Medical Research, The University of Sydney, Westmead Hospital, Westmead, Australia.
PLoS One. 2017 Mar 8;12(3):e0172528. doi: 10.1371/journal.pone.0172528. eCollection 2017.
Diarrhoeal diseases in piglets caused by Escherichia coli are responsible for substantial losses each year in the Australian pig industry. A cross-sectional survey was conducted (September 2013-May 2014) across 22 commercial pig herds located in southeastern Australia: NSW (n = 9); VIC (n = 10); and SA (n = 3), to estimate the prevalence of E. coli associated diarrhoea in pre- and post-weaned piglets and to identify key risk factors associated with E. coli disease. A questionnaire on management and husbandry practices was included. Faecal samples (n = 50 from each herd) were tested for the presence of β-haemolytic E. coli. Species level identification was confirmed by matrix-assisted laser desorption / ionisation time-of-flight mass spectrometry (MALDI-TOF/MS). ETEC virulence and enterotoxin genes (F4, F5, F6, F18, F41, STa, STb and LT) were screened for by multiplex PCR. This study assessed 60 potential risk factors for E. coli disease in post-weaned piglets, with 2 key factors-recent disease events and the presence of bedding, statistically associated with the presence of post-weaning scours. The prevalence of diarrhea in pre-weaned pens was 17% (16/93), compared with 24% (24/102) in post-weaned pens. The most prevalent β-haemolytic ETEC genes were F18 (32%) and STb (32%) but isolates were more likely to contain F4:STb (11 of 22 herds, 23%), than F18:STb (5 of 22 herds, 6%). These findings indicate that recent disease events that have occurred within the last 12 months, and by the use of bedding or not maintaining fresh bedding can have significant impacts on piglet diarrhoea.
在澳大利亚养猪业中，由大肠杆菌引起的仔猪腹泻病每年都会造成巨大损失。于2013年9月至2014年5月对位于澳大利亚东南部的22个商业猪群进行了横断面调查，这些猪群分布在新南威尔士州（n = 9）、维多利亚州（n = 10）和南澳大利亚州（n = 3），以估计断奶前和断奶后仔猪中与大肠杆菌相关腹泻的患病率，并确定与大肠杆菌病相关的关键风险因素。其中包含了一份关于管理和饲养方式的问卷。对粪便样本（每个猪群50份）进行β - 溶血大肠杆菌检测。通过基质辅助激光解吸/电离飞行时间质谱（MALDI - TOF/MS）确认菌种水平鉴定。通过多重PCR筛选产肠毒素大肠杆菌（ETEC）毒力和肠毒素基因（F4、F5、F6、F18、F41、STa、STb和LT）。本研究评估了断奶后仔猪大肠杆菌病的60个潜在风险因素，其中有2个关键因素——近期疾病事件和垫料的存在，与断奶后腹泻的发生在统计学上相关。断奶前猪栏中腹泻的患病率为17%（16/93），断奶后猪栏中为24%（24/102）。最常见的β - 溶血ETEC基因是F18（32%）和STb（32%），但分离株更可能含有F4:STb（22个猪群中的11个，23%），而非F18:STb（22个猪群中的5个，6%）。这些发现表明，过去12个月内发生的近期疾病事件以及是否使用垫料或未保持垫料新鲜，可能对仔猪腹泻产生重大影响。
