School of Sports Science, Beijing Sport University, Beijing 100084, China.
China Institute of Sports and Health, Beijing Sport University, Beijing 100084, China.
Neural Plast. 2022 Oct 19;2022:7382327. doi: 10.1155/2022/7382327. eCollection 2022.
Peripheral nerve injury leads to severe neuropathic pain. Previous studies have highlighted the beneficial effects of physical exercise on alleviating neuropathic pain. Exercise regulating transforming growth factor-1 (TGF-1) can improve several diseases and relieve neuropathic pain induced by peripheral nerve injury. Here, we investigated whether exercise could alleviate neuropathic pain by modulating TGF-1 expression. We assessed mechanical and cold pain behavior and conducted molecular evaluation of the spinal cord. We found that spared nerve injury (SNI) led to mechanical and cold allodynia in the hind paw, elevated the expression of latency-associated peptide- (LAP-) TGF-1, and activated astroglial in the spinal cord. Exercise decreases allodynia, astroglial activation, and LAP-TGF-1 in SNI mice. Intrathecal injection of a TGF-type I receptor inhibitor attenuated exercise analgesia and enhanced astroglial activation. These findings demonstrate that exercise induces analgesia by promoting TGF-1 activation and inhibiting astrogliosis. Our study reveals a new underlying mechanism for exercise-attenuated neuropathic pain in the maintenance stage of neuropathic pain after nerve injury.
周围神经损伤导致严重的神经性疼痛。先前的研究强调了体育锻炼对缓解神经性疼痛的有益作用。运动调节转化生长因子-1（TGF-1）可以改善多种疾病，并缓解周围神经损伤引起的神经性疼痛。在这里，我们研究了运动是否可以通过调节 TGF-1 的表达来缓解神经性疼痛。我们评估了机械性和冷痛行为，并对脊髓进行了分子评估。我们发现， spared nerve injury（SNI）导致后爪的机械性和冷性痛觉过敏， latency-associated peptide-（LAP-）TGF-1 的表达增加，并激活脊髓中的星形胶质细胞。运动可减轻 SNI 小鼠的痛觉过敏、星形胶质细胞激活和 LAP-TGF-1。鞘内注射 TGF 型 I 受体抑制剂减弱了运动镇痛作用，并增强了星形胶质细胞激活。这些发现表明，运动通过促进 TGF-1 的激活和抑制星形胶质细胞增生来诱导镇痛。我们的研究揭示了神经损伤后神经性疼痛维持阶段运动减轻神经性疼痛的一个新的潜在机制。
