Li Nuomin, Wang Hao, Xin Shuchen, Min Rui, Zhang Yongqian, Deng Yulin
Institute of Engineering Medicine, Beijing Institute of Technology, Beijing, China.
School of Life Science, Beijing Institute of Technology, Beijing, China.
Front Physiol. 2022 Nov 24;13:999574. doi: 10.3389/fphys.2022.999574. eCollection 2022.
A confined environment is an enclosed area where entry or exit is highly restricted, which is a risk factor for a work crew's mental health. Previous studies have shown that a crew is more susceptible to developing anxiety or depression in a confined environment. However, the underlying mechanism by which negative emotion is induced by confinement is not fully understood. Hence, in this study, mice were retained in a tube to simulate short-term confinement. The mice exhibited depressive-like behavior. Additionally, the levels of HO and malondialdehyde in the prefrontal cortex were significantly increased in the confinement group. Furthermore, a label-free quantitative proteomic strategy was applied to analyze the abundance of proteins in the prefrontal cortex of mice. A total of 71 proteins were considered differentially abundant proteins among 3,023 identified proteins. Two differentially abundant proteins, superoxide dismutase [Mn] and syntaxin-1A, were also validated by a parallel reaction monitoring assay. Strikingly, the differentially abundant proteins were highly enriched in the respiratory chain, oxidative phosphorylation, and the synaptic vesicle cycle, which might lead to oxidative damage and synaptic dysfunction. The results of this study provide valuable information to better understand the mechanisms of depressive-like behavior induced by confined environments.
密闭环境是指进出受到严格限制的封闭区域，这是影响工作人员心理健康的一个风险因素。先前的研究表明，工作人员在密闭环境中更容易出现焦虑或抑郁情绪。然而，目前对于禁闭诱发负面情绪的潜在机制尚不完全清楚。因此，在本研究中，将小鼠置于管中以模拟短期禁闭。小鼠表现出类似抑郁的行为。此外，禁闭组小鼠前额叶皮质中HO和丙二醛的水平显著升高。此外，采用无标记定量蛋白质组学策略分析小鼠前额叶皮质中蛋白质的丰度。在3023种已鉴定的蛋白质中，共有71种蛋白质被认为是差异丰富蛋白质。通过平行反应监测分析还验证了两种差异丰富蛋白质，即超氧化物歧化酶[Mn]和 syntaxin-1A。值得注意的是，差异丰富蛋白质在呼吸链、氧化磷酸化和突触小泡循环中高度富集，这可能导致氧化损伤和突触功能障碍。本研究结果为更好地理解密闭环境诱发类似抑郁行为的机制提供了有价值的信息。
