Institute of Chinese Materia Medica, China Academy of Chinese Medical Sciences, Beijing, China.
Institute of Traditional Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin, China.
Front Cell Infect Microbiol. 2022 Nov 23;12:1004751. doi: 10.3389/fcimb.2022.1004751. eCollection 2022.
The seeds of (Neck.) are from a traditional medicinal plant Garcke, also called Wang-Bu-Liu-Xing in China. According to the Chinese Pharmacopoeia, the seeds of can be used for treating urinary system diseases. This study was designed to investigate the underlying mechanism of VSP (polysaccharides from ) against urinary tract infections caused by uropathogenic (UPEC). Here, both and infection models were established with the UPEC strain CFT073. Bacterial adhesion and invasion into bladder epithelial cells were analyzed. We found that VSP reduced the adhesion of UPEC to the host by inhibiting the expression of bacterial hair follicle adhesion genes. VSP also reduced the invasion of UPEC by regulating the uroplakins and Toll-like receptors of host epithelial cells. In addition, the swarming motility and flagella-mediated motility genes flhC, flhD and Flic of UPEC were diminished after VSP intervention. Taken together, our findings reveal a possible mechanism by which VSP interferes with the adhesion and invasion of UPEC.
（Neck.）的种子来自一种传统的药用植物 Garcke，在中国也被称为 Wang-Bu-Liu-Xing。根据《中国药典》，可以使用来治疗泌尿系统疾病。本研究旨在探讨 VSP（来自 ）对尿路致病性大肠杆菌（UPEC）引起的尿路感染的潜在机制。在这里，使用 UPEC 菌株 CFT073 建立了 和 感染模型。分析了细菌黏附和侵袭膀胱上皮细胞的情况。我们发现，VSP 通过抑制细菌发状附属物黏附基因的表达，减少 UPEC 与宿主的黏附。VSP 还通过调节宿主上皮细胞的尿路上皮蛋白和 Toll 样受体，减少 UPEC 的侵袭。此外，VSP 干预后 UPEC 的群集运动和鞭毛介导的运动基因 flhC、flhD 和 Flic 减少。总之，我们的研究结果揭示了 VSP 干扰 UPEC 黏附和侵袭的可能机制。
