非手术牙周治疗对慢性牙周炎患者龈沟液中五聚体-3浓度的影响：一项临床试验
The Effect of Non-Surgical Periodontal Therapy on Pentraxin-3 Concentration in Gingival Crevicular Fluid of Chronic Periodontitis Patients: A Clinical Trial.
作者信息
Mozaffari Asieh, Samieifar Mahtab, Abd Nikfarjam Bahare, Mirzadeh Monirsadat, Assareh Shadi, Mirzapour Samira Mohammad
机构信息
Dept. of Periodontology, Dental Caries Prevention Research Center, Qazvin University of Medical Sciences, Qazvin, Iran.
Dept. of Immunology, School of Medicine, Qazvin University of Medical Sciences, Qazvin, Iran.
出版信息
J Dent (Shiraz). 2022 Sep;23(3):314-320. doi: 10.30476/DENTJODS.2021.90647.1510.
STATEMENT OF THE PROBLEM
Periodontitis is a multifactorial disease caused by periopathogens and its severity is determined by the host immune response. Gingival crevicular fluid (GCF) can be used for non-invasive testing to assess the host response in periodontal treatment. Pentraxins are the classic mediators of inflammation and pentraxin-3 can be used as a marker to assess response to therapy, which was investigated in this study.
PURPOSE
This study aimed to assess the effect of non-surgical periodontal therapy on GCF level of pentraxin-3 in patients with chronic periodontitis.
MATERIALS AND METHOD
25 patients with chronic periodontitis (CP) and 25 periodontally healthy controls were evaluated. Pocket probing depth, clinical attachment loss, plaque index, gingival index, and bleeding on probing were measured in both groups. GCF samples were collected using paper strips to assess the level of pentraxin-3. In the CP group, GCF samples were collected from the highest clinical attachment loss, pocket probing depth, and bone loss at baseline and six weeks after non-surgical therapy. The level of pentraxin-3 in the GCF was quantified by enzyme-linked immunosorbent assay (ELISA). Data were analyzed using SPSS version 23.
RESULTS
Pentraxin-3 in GCF of CP patients before treatment (6.72±4.63 ng/mL) was higher than the control group (4.43±2.85 ng/mL). Pentraxin-3 in patients after non-surgical therapy (3.2±2.66 ng/mL) decreased significantly compared to the baseline (= 0.04) and its level after treatment was not significantly different from the control group (= 0.14).
CONCLUSION
Pentraxin-3 in GCF of CP patients was higher than healthy controls and decreased in response to non-surgical periodontal therapy. Thus, it can be used as an inflammatory marker for detection of patients at risk of CP. However, further studies with larger samples and longer follow-ups in different populations are required to confirm our findings.
问题陈述
牙周炎是一种由牙周病原体引起的多因素疾病，其严重程度由宿主免疫反应决定。龈沟液（GCF）可用于非侵入性检测，以评估牙周治疗中的宿主反应。五聚素是炎症的经典介质，五聚素-3可作为评估治疗反应的标志物，本研究对此进行了调查。
目的
本研究旨在评估非手术牙周治疗对慢性牙周炎患者龈沟液中五聚素-3水平的影响。
材料与方法
对25例慢性牙周炎（CP）患者和25例牙周健康对照者进行评估。测量两组的牙周袋探诊深度、临床附着丧失、菌斑指数、牙龈指数和探诊出血情况。使用纸条收集GCF样本，以评估五聚素-3水平。在CP组中，在基线时以及非手术治疗后6周，从临床附着丧失、牙周袋探诊深度和骨丧失最严重处收集GCF样本。通过酶联免疫吸附测定（ELISA）对GCF中五聚素-3的水平进行定量。使用SPSS 23版软件对数据进行分析。
结果
CP患者治疗前GCF中的五聚素-3（6.72±4.63 ng/mL）高于对照组（4.43±2.85 ng/mL）。非手术治疗后患者的五聚素-3（3.2±2.66 ng/mL）与基线相比显著降低（P = 0.04），且治疗后的水平与对照组无显著差异（P = 0.14）。
结论
CP患者GCF中的五聚素-3高于健康对照者，并在非手术牙周治疗后降低。因此，它可作为检测CP高危患者的炎症标志物。然而，需要在不同人群中进行更大样本量和更长随访时间的进一步研究来证实我们的发现。
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