Kido J, Bando Y, Bando M, Kajiura Y, Hiroshima Y, Inagaki Y, Murata H, Ikuta T, Kido R, Naruishi K, Funaki M, Nagata T
Department of Periodontology and Endodontology, Institute of Health Bioscience, The University of Tokushima Graduate School, Tokushima, Japan.
Clinical Research Center for Diabetes, Tokushima University Hospital, Tokushima, Japan.
Oral Dis. 2015 Jul;21(5):667-73. doi: 10.1111/odi.12334. Epub 2015 Apr 6.
YKL-40 is a chitin-binding glycoprotein, the level of which increases in inflammatory diseases, diabetes mellitus (DM), cardiovascular diseases, and tumors. Gingival crevicular fluid (GCF) contains many proteins and markers of periodontitis. The purpose of this study was to investigate YKL-40 level in GCF from patients with periodontitis and DM and the association between YKL-40 level and chronic periodontitis (CP) or DM.
The subjects were 121 patients with DM, CP, DM and periodontitis (DM-P), and healthy subjects (H). GCF was collected using paper strips after the sites for GCF collection were clinically evaluated for probing depth (PD), gingival index (GI), and bleeding on probing (BOP). YKL-40 in GCF was identified by Western blotting, and its level was determined by ELISA.
YKL-40 was contained in GCF samples from H, DM, CP, and DM-P sites, and its levels (amount and concentration) in CP and DM-P were significantly higher than those in H and DM. GCF YKL-40 level significantly correlated with PD and GI, and its level in BOP-positive sites was significantly higher than that in BOP-negative ones.
GCF YKL-40 level was elevated in periodontitis, but not DM. YKL-40 in GCF may be an inflammatory marker for periodontitis.
YKL - 40是一种几丁质结合糖蛋白，其水平在炎症性疾病、糖尿病（DM）、心血管疾病和肿瘤中会升高。龈沟液（GCF）含有多种蛋白质和牙周炎标志物。本研究的目的是调查牙周炎合并糖尿病患者龈沟液中YKL - 40的水平，以及YKL - 40水平与慢性牙周炎（CP）或糖尿病之间的关联。
研究对象为121例糖尿病患者、慢性牙周炎患者、糖尿病合并牙周炎（DM - P）患者以及健康受试者（H）。在对龈沟液采集部位进行临床评估，测量探诊深度（PD）、牙龈指数（GI）和探诊出血（BOP）后，使用纸条收集龈沟液。通过蛋白质免疫印迹法鉴定龈沟液中的YKL - 40，并采用酶联免疫吸附测定法测定其水平。
在健康受试者、糖尿病患者、慢性牙周炎患者和糖尿病合并牙周炎患者的龈沟液样本中均检测到YKL - 40，慢性牙周炎患者和糖尿病合并牙周炎患者龈沟液中YKL - 40的水平（含量和浓度）显著高于健康受试者和糖尿病患者。龈沟液YKL - 40水平与探诊深度和牙龈指数显著相关，探诊出血阳性部位的YKL - 40水平显著高于探诊出血阴性部位。
牙周炎患者龈沟液中YKL - 40水平升高，而糖尿病患者未升高。龈沟液中的YKL - 40可能是牙周炎的一种炎症标志物。
