Turgut Çankaya Zeynep, Bodur Ayşen, Taçoy Gülten, Ergüder Imge, Aktuna Derya, Çengel Atiye
Gazi University, Faculty of Dentistry, Department of Periodontology, Ankara, Turkey.
Gazi University, Faculty of Medicine, Department of Cardiology, Ankara, Turkey.
J Appl Oral Sci. 2018 Apr 5;26:e20170199. doi: 10.1590/1678-7757-2017-0199.
The presence of neopterin in gingival crevicular fluid (GCF) is a marker for local and acute immune activation, and the presence of vascular cell adhesion molecule (VCAM-1) in GCF is accepted as a marker for chronic vascular inflammation.
This study aimed to evaluate effects of periodontal treatment on GCF levels of neopterin and VCAM-1 in patients with chronic periodontitis (CP) with acute myocardial infarction (AMI) compared with systemically healthy CP patients.
Sixty subjects (20 CP patients with AMI, 20 healthy CP patients, and 20 healthy controls) were included. GCF samples were analyzed at baseline and after 3 and 6 months, and the probing pocket depth (PD), clinical attachment level (CAL), bleeding on probing, gingival (GI) and plaque (PI) indices were recorded. We determined neopterin and VCAM-1 levels (concentration and total amount) using enzyme-linked immunosorbent assay (ELISA). No significant differences were seen between the AMI+CP and CP groups for PI, GI, GCF levels of neopterin and VCAM-1 at baseline.
The number of teeth with 5 mm≤CAL<7 mm and CAL≥7 mm were significantly increased in the AMI+CP group at baseline. There were no significant differences between the AMI+CP and CP for PI, CAL, GCF volumes, and the AMI+CP group had the highest clinical improvement in the number of teeth with 5 mm≤CAL<7 mm at the sixth month. There were significant positive correlations between clinical periodontal inflammation and the presence of neopterin and VCAM-1 in GCF prior to and following periodontal treatment, and between the GCF volume and clinical parameters.
Data suggest that the total amount and concentration of neopterin and VCAM-1 in GCF seemed to be closely associated with periodontal disease severity in CP patients with AMI. Moreover, the results of our study demonstrate that the past periodontal status is potentially correlated between groups, with similar periodontal disease severity.
龈沟液（GCF）中蝶呤的存在是局部和急性免疫激活的标志物，GCF中血管细胞粘附分子（VCAM-1）的存在被认为是慢性血管炎症的标志物。
本研究旨在评估与全身健康的慢性牙周炎（CP）患者相比，牙周治疗对合并急性心肌梗死（AMI）的CP患者GCF中蝶呤和VCAM-1水平的影响。
纳入60名受试者（20名合并AMI的CP患者、20名健康的CP患者和20名健康对照）。在基线以及3个月和6个月后分析GCF样本，并记录探诊深度（PD）、临床附着水平（CAL）、探诊出血、牙龈（GI）和菌斑（PI）指数。我们使用酶联免疫吸附测定（ELISA）测定蝶呤和VCAM-1水平（浓度和总量）。在基线时，AMI+CP组和CP组之间在PI、GI、GCF中蝶呤和VCAM-1水平方面未见显著差异。
基线时，AMI+CP组中CAL为5 mm≤CAL<7 mm和CAL≥7 mm的牙齿数量显著增加。AMI+CP组和CP组在PI、CAL、GCF量方面无显著差异，且AMI+CP组在第6个月时CAL为5 mm≤CAL<7 mm的牙齿数量临床改善最大。在牙周治疗前后，临床牙周炎症与GCF中蝶呤和VCAM-1的存在之间，以及GCF量与临床参数之间存在显著正相关。
数据表明，GCF中蝶呤和VCAM-1的总量和浓度似乎与合并AMI的CP患者的牙周疾病严重程度密切相关。此外，我们的研究结果表明，过去的牙周状况在各组之间可能相关，牙周疾病严重程度相似。
