Universidade Federal de Uberlândia - UFU, School of Dentistry, Department of Endodontics, Uberlândia, MG, Brazil.
Universidade Estadual Paulista - Unesp, School of Dentistry, Department of Restorative Dentistry, Araraquara, SP, Brazil.
Braz Oral Res. 2022 May 2;36:e065. doi: 10.1590/1807-3107bor-2022.vol36.0065. eCollection 2022.
Solubility, pH, ion release, cytotoxicity, and osteoclastogenesis inhibition in bone marrow-derived monocyte macrophages (BMMs) were evaluated in EndoSequence BC Sealer (END), Bio-C Sealer (BC), and Sealer Plus BC (SPBC). pH was determined after immersion of the sealers in deionized water (DW) and Minimum Essential Medium Alpha (α-MEM). Solubility was obtained by mass loss. Ion release was measured by using X-ray fluorescence spectroscopy (XRF). Cytotoxicity was evaluated by MTT assay. Inhibition of osteoclastogenesis was evaluated by tartrate-resistant acid phosphatase (TRAP). Data were analyzed using the t-test, ANOVA and Tukey/Dunnett's post-hoc tests (α = 0.05). END had the highest pH in DW (p < 0.05), and BC, in α-MEM (p < 0.05). Solubility in DW was the lowest for SPBC (p < 0.005). The highest calcium release was observed for BC in DW at 12 h (p < 0.05), and in α-MEM at 12 and 24 h (p < 0.05). The lowest toxicity was detected for END (p < 0.05). BC had the highest inhibitory effect on osteoclasts (p < 0.05). Overall, the highest solubility and pH values were found in DW. However, the calcium silicate-based sealer showed higher solubility than the ISO standards. Calcium release was the highest for BC. END showed the highest cell viability, and BC, the highest osteoclast inhibition.
在骨髓来源的单核巨噬细胞（BMMs）中评估了 EndoSequence BC 密封剂（END）、Bio-C 密封剂（BC）和 Sealer Plus BC（SPBC）的溶解度、pH 值、离子释放、细胞毒性和破骨细胞生成抑制作用。将密封剂浸入去离子水（DW）和最小必需培养基 Alpha（α-MEM）中后测定 pH 值。通过质量损失获得溶解度。通过 X 射线荧光光谱法（XRF）测量离子释放。通过 MTT 测定法评估细胞毒性。通过抗酒石酸酸性磷酸酶（TRAP）评估破骨细胞生成抑制作用。使用 t 检验、方差分析和 Tukey/Dunnett 事后检验（α=0.05）分析数据。在 DW 中，END 的 pH 值最高（p<0.05），而 BC 的 pH 值在 α-MEM 中最高（p<0.05）。在 DW 中，SPBC 的溶解度最低（p<0.005）。在 DW 中，BC 在 12 小时时观察到最高的钙释放（p<0.05），在 α-MEM 中在 12 和 24 小时时观察到最高的钙释放（p<0.05）。检测到 END 的毒性最低（p<0.05）。BC 对破骨细胞的抑制作用最强（p<0.05）。总体而言，在 DW 中发现最高的溶解度和 pH 值。然而，基于硅酸钙的密封剂的溶解度高于 ISO 标准。BC 的钙释放最高。END 显示出最高的细胞活力，而 BC 显示出最高的破骨细胞抑制作用。
