Kalén A, Norling B, Appelkvist E L, Dallner G
Department of Pathology, Huddinge Hospital, Karolinska Institutet, Stockholm, Sweden.
Biochim Biophys Acta. 1987 Oct 8;926(1):70-8. doi: 10.1016/0304-4165(87)90183-8.
The distribution and biosynthesis of ubiquinone were investigated in vivo in rats and using liver slices. In addition to mitochondria, Golgi vesicles and lysosomes also contain large amounts of this lipid, and even the plasma membrane, peroxisomes and microsomes demonstrate easily measurable amounts. The spectral and chromatographic properties of microsomal ubiquinone were identical to those of its mitochondrial counterpart. When pentane was used to deplete beef heart submitochondrial particles of ubiquinone, NADH and succinate oxidase activities could be restored by reincorporation of microsomal ubiquinone. Injection of [3H]mevalonate into the portal vein of rats and incubation of liver slices with [3H]mevalonate and [3H]- and [14C]tyrosine demonstrated that labeling of mitochondrial ubiquinone was initially much lower than labeling of the microsomal lipid. Furthermore, intraportal injection of [3H]mevalonate resulted in the rapid appearance of labeled ubiquinone in the blood. These results indicate that ubiquinone is synthesized not only in mitochondria, but also on the endoplasmic reticulum of rat liver.
在大鼠体内以及使用肝切片对泛醌的分布和生物合成进行了研究。除线粒体之外，高尔基体囊泡和溶酶体也含有大量这种脂质，甚至质膜、过氧化物酶体和微粒体也显示出可轻松测量的量。微粒体泛醌的光谱和色谱特性与其线粒体对应物相同。当使用戊烷耗尽牛心亚线粒体颗粒中的泛醌时，通过重新掺入微粒体泛醌可恢复NADH和琥珀酸氧化酶活性。将[3H]甲羟戊酸注射到大鼠门静脉中，并用[3H]甲羟戊酸以及[3H] - 和[14C]酪氨酸孵育肝切片，结果表明线粒体泛醌的标记最初远低于微粒体脂质的标记。此外，门静脉内注射[3H]甲羟戊酸导致血液中迅速出现标记的泛醌。这些结果表明，泛醌不仅在线粒体中合成，也在大鼠肝脏的内质网上合成。
