Department of Pharmaceutical Analysis, Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, The School of Pharmacy, Fujian Medical University, Fuzhou, 350122, China.
Department of Pharmaceutical Analysis, Higher Educational Key Laboratory for Nano Biomedical Technology of Fujian Province, The School of Pharmacy, Fujian Medical University, Fuzhou, 350122, China.
Biosens Bioelectron. 2023 Feb 15;222:115001. doi: 10.1016/j.bios.2022.115001. Epub 2022 Dec 9.
P-glycoprotein (P-gp), a transmembrane glycoprotein widely expressed on the surface of various cells, is highly associated with multidrug resistance (MDR) that heralds the malignant progress of disease after drug treatment. Notably, there have been reported that serum P-gp is a potential marker for assessing the progression of disease resistance. Currently, there are few methods for point-of-care serum P-gp detection. In this study, we proposed a gold nanoparticles/electrochemically reduced graphene oxide@carbon nanotube (AuNPs/ERGO@CNT) modified immunosensor based on a one-step electrochemical co-reduction method. The limit of detection (LOD) of our constructed electrochemical immunosensor for P-gp detection reached 0.13 ng/mL, and the detection results in serum were consistent with ELISA. The developed immunosensor is expected to provide a scientific basis for the clinical application of serum P-gp monitoring and integrated medicine.
P-糖蛋白（P-gp）是一种广泛表达于各种细胞表面的跨膜糖蛋白，与多药耐药（MDR）高度相关，预示着药物治疗后疾病的恶性进展。值得注意的是，已有报道称血清 P-gp 是评估疾病耐药进展的潜在标志物。目前，用于即时检测血清 P-gp 的方法较少。本研究提出了一种基于一步电化学共还原法的金纳米粒子/电化学还原氧化石墨烯@碳纳米管（AuNPs/ERGO@CNT）修饰免疫传感器。所构建的电化学免疫传感器用于 P-gp 检测的检测限（LOD）达到 0.13ng/mL，且血清中的检测结果与 ELISA 一致。该免疫传感器有望为血清 P-gp 监测和整合医学的临床应用提供科学依据。
