Fu Xuhuai, Gao Ke, Liu Nanjing, Guo Bianqin, He Meng, Lai Nianyu, Li Xinyu, Ding Shijia, He Xiaoyan, Wu Lixiang
Department of Clinical Laboratory, Chongqing Key Laboratory of Translational Research for Cancer Metastasis and Individualized Treatment, Chongqing University Cancer Hospital and Chongqing Cancer Institute, Chongqing, 400030, China.
Department of Laboratory Medicine, Chonggang General Hospital, Chongqing, 400080, People's Republic of China.
Mikrochim Acta. 2024 Feb 8;191(3):126. doi: 10.1007/s00604-023-06168-1.
An ultrasensitive sandwich-type electrochemical immunosensor for pro-gastrin-releasing peptide (ProGRP) detection was constructed based on PtCu nanodendrites functionalized Au/polyaniline nanospheres (Au/PANI@PtCu). The prepared Au/PANI@PtCu nanocomposites not only possessed excellent electro-catalytic activity of HO reduction due to the synergistic effect between the Au/PANI and PtCu NDs but also provided large specific surface area for detection of antibodies (Ab) immobilization. In addition, Au nanoparticles encapsulated multi-wall carbon nanotubes (AuNPs@MWCNTs) were also applied to modify the glassy carbon electrode interface for loading numerous capture antibodies (Ab). In the presence of target ProGRP, a sandwich-type electrochemical immunosensor showed a strong current response from the electro-catalysis of Au/PANI@PtCu toward HO reduction. Benefiting from the exceptional electro-catalytic performance of Au/PANI@PtCu and the high conductivity of AuNPs@MWCNTs, the sandwich-type immunoassay exhibited remarkable sensitivity in detection. The linear range extended from 100 fg/mL to 10 ng/mL, while achieving an impressively low limit of detection of 77.62 fg/mL.
基于功能化的金/聚苯胺纳米球（Au/PANI）修饰的铂铜纳米枝晶（PtCu）构建了一种用于检测胃泌素释放肽前体（ProGRP）的超灵敏夹心型电化学免疫传感器。所制备的Au/PANI@PtCu纳米复合材料不仅由于Au/PANI与PtCu纳米枝晶之间的协同效应而具有优异的电催化还原过氧化氢的活性，而且为检测抗体（Ab）的固定提供了较大的比表面积。此外，金纳米颗粒包裹的多壁碳纳米管（AuNPs@MWCNTs）也被用于修饰玻碳电极界面，以负载大量的捕获抗体（Ab）。在存在目标ProGRP的情况下，夹心型电化学免疫传感器对Au/PANI@PtCu催化还原过氧化氢产生强烈的电流响应。得益于Au/PANI@PtCu出色的电催化性能和AuNPs@MWCNTs的高导电性，夹心型免疫分析在检测中表现出显著的灵敏度。线性范围从100 fg/mL扩展到10 ng/mL，同时实现了低至77.62 fg/mL的检测限。
