Li Siqi, Huang Congying, Li Xing, Meng Xiangxi, Wen Rong, Zhang Xiaodong, Zhang Chunhong, Li Minhui
Baotou Medical College, Baotou 014040, China.
Inner Mongolia Autonomous Region Hospital of Traditional Chinese Medicine, Hohhot 010020, China.
Toxicol Rep. 2022 Aug 17;9:1655-1665. doi: 10.1016/j.toxrep.2022.08.006. eCollection 2022.
Cardiovascular disease is the most common disease in the world and the first among the causes of human death. Its morbidity and mortality increase annually, but no effective treatment is available. Therefore, new drugs should be developed to treat cardiovascular disease. (Michx.) Hulten () is an important Mongolian medicine in China and elicits protective effects on cardiovascular health. In this study, liquid chromatography-mass spectrometry (LC-MS) combined with network pharmacology was used to screen the main active ingredients and confirm that bellidifolin was one of the main components for the treatment of ischemic heart disease. Then, rat myocardial (H9c2) cells injury model induced by hydrogen peroxide (HO) was established to verify the effect of bellidifolin on oxidative stress stimulation, including determination of antioxidant enzyme activity and apoptosis. Transcriptome sequencing, qRT-PCR, and western blot were performed to further verify the antioxidant stress mechanism of bellidifolin. Results showed that bellidifolin pretreatment decreased the rate of apoptosis and the levels of lactate dehydrogenase (LDH), creatine kinase (CK), and alanine aminotransferase (ALT). Conversely, it increased the contents of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) in a dose-dependent manner, indicating that bellidifolin caused a protective effect on cardiomyocyte injury. Bellidifolin minimized the HO-induced cell injury by activating the PI3K-Akt signal pathway and downregulating glycogen synthase kinase-3β (GSK-3β) and p-Akt1/Akt1. Therefore, this work revealed that has a good development prospect as an edible medicinal plant in cardiovascular disease. Its bellidifolin component is a potential therapeutic agent for cardiovascular disease induced by oxidative stress damage.
心血管疾病是世界上最常见的疾病，也是人类死亡原因之首。其发病率和死亡率逐年上升，但尚无有效的治疗方法。因此，应研发治疗心血管疾病的新药。（米克斯）胡尔滕（）是中国一种重要的蒙药，对心血管健康具有保护作用。在本研究中，采用液相色谱 - 质谱联用（LC - MS）结合网络药理学筛选主要活性成分，并证实灯盏乙素是治疗缺血性心脏病的主要成分之一。然后，建立过氧化氢（HO）诱导的大鼠心肌（H9c2）细胞损伤模型，以验证灯盏乙素对氧化应激刺激的影响，包括测定抗氧化酶活性和细胞凋亡情况。进行转录组测序、qRT - PCR和蛋白质印迹法以进一步验证灯盏乙素的抗氧化应激机制。结果表明，灯盏乙素预处理降低了细胞凋亡率以及乳酸脱氢酶（LDH）、肌酸激酶（CK）和谷丙转氨酶（ALT）的水平。相反，它以剂量依赖的方式增加了超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH - Px）的含量，表明灯盏乙素对心肌细胞损伤具有保护作用。灯盏乙素通过激活PI3K - Akt信号通路并下调糖原合酶激酶 - 3β（GSK - 3β）和p - Akt1/Akt1，使HO诱导的细胞损伤最小化。因此，这项研究表明，作为一种可食用的药用植物，在心血管疾病治疗方面具有良好的发展前景。其灯盏乙素成分是氧化应激损伤诱导的心血管疾病的潜在治疗药物。
